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    Recombinant Mouse anti-Human ENO1 Monoclonal antibody (3H3BH22CG9) (CAT#: MOB-0661CT)

    Recombinant Mouse monoclonal antibody to Human ENO1, expressed in Chinese Hamster Ovary cells(CHO).

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    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68284/1285_E3_4_selected.jpg
    Figure 2 IF staining of human cell line A-431
    Figure 2 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/68721/1717_C1_35_selected.jpg
    Figure 3 IF staining of human cell line A549
    Figure 3 IF staining of human cell line A549
    (Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/990_G8_1_selected.jpg
    Figure 4 IHC staining of human pancreas
    Figure 4 IHC staining of human pancreas
    (Immunohistochemical staining of human pancreas shows distinct positivity in intercalated ducts.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/ihc_selected.jpg
    Figure 5 IHC staining of human kidney
    Figure 5 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows moderate positivity.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/ihc_selected.jpg
    Figure 6 IHC staining of human kidney
    Figure 6 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80034/170663_A_7_5_selected.jpg
    Figure 7 IF staining of human cell line MCF7
    Figure 7 IF staining of human cell line MCF7
    (Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18614/962_H4_1_blue_red_green.jpg
    Normal Tissue
    Figure 8 Cerebral cortex
    Figure 8 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuronal cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_B_8_5.jpg
    Figure 9 Colon
    Figure 9 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_3.jpg
    Figure 10 Liver
    Figure 10 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Hepatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_4.jpg
    Figure 11 Pancreas
    Figure 11 Pancreas
    (Exocrine glandular cells
    Staining:High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_1_3.jpg
    Figure 12 Kidney
    Figure 12 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_8_5.jpg
    Figure 13 Testis
    Figure 13 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_5_6.jpg
    Figure 14 Lymph node
    Figure 14 Lymph node
    (Germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Non-germinal center cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/69394/150509_A_7_8.jpg
    RNA Expression
    Figure 15 RNA cell line category: Low cell line specificity
    Figure 15 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000074800-ENO1

    Specifications

    • Immunogen
    • Hela cell fraction
    • Host Species
    • Mouse
    • Specificity
    • Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
    • Species Reactivity
    • Human
    • Clone
    • 3H3BH22CG9
    • Applications
    • Used for immunoassay techniques such as: Flow Cytometry; Immunocytochemistry/Immunofluorescence; Immunoprecipitation; Enzyme-linked Immunosorbent Assay
    • Conjugate
    • Unconjugated

    Product Property

    • Purity
    • Immunogen affinity purified
    • Format
    • Liquid
    • Concentration
    • 1.0 mg/ml
    • Buffer
    • Preservative:0.02% Sodium azideConstituent:99.98% HBS
    • Storage
    • Store at +4°C. Do not freeze.

    Target

    • Alternative Names
    • ENO1; enolase 1, (alpha); NNE; PPH; MPB1; ENO1L1; alpha-enolase; enolase-alpha; tau-crystallin; non-neural enolase; alpha enolase like 1; phosphopyruvate hydratase; plasminogen-binding protein; MYC promoter-binding protein 1; 2-phospho-D-glycerate hydro-lyase;
    • Function
    • DNA binding; lyase activity; magnesium ion binding; phosphopyruvate hydratase activity; protein binding; sequence-specific DNA binding transcription factor activity; transcription corepressor activity;

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

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    CAT Product Name Application Type
    MOB-1162z Mouse Anti-ENO1 Recombinant Antibody (clone 28F5) WB, ELISA, IF, IHC Mouse IgG1, κ
    HPAB-0054-CN Mouse Anti-ENO1 Recombinant Antibody (HPAB-0054-CN) FC, ELISA Mouse IgG
    HPAB-0055-CN Human Anti-ENO1 Recombinant Antibody (HPAB-0055-CN) FC, ELISA Humanized IgG
    VS3-WK1658 Recombinant Mouse Anti-ENO1 Capture Antibody (clone 9F10) ELISA Mouse IgG2
    VS3-WK1659 Recombinant Mouse Anti-ENO1 Detection Antibody (clone 6H2) ELISA Mouse IgG1

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