Anti-Human ALPP Recombinant Antibody (MS17-57)
CAT#: TAB-0300CL
The anti-cell surface ectopic expression monoclonal antibody MS17-57 has an affinity for placental ALP (PALP), which was found increasing in hepatobiliary system diseases. MS17-57 can be used for treating and preventing digestive tract tumors or digestive tract tumor metastasis.
Published Data
Gene Expression
Inhib
Figure 1 MS17-57 inhibits BGC823 cell growth.
MS17-57 was added to BGC823 cells (8 µg/mL per well) on 96-well tissue culture plates at day 0. MS17-57 inhibited BGC823 cell growth by about 27.5% for up to 5 days. Irrelevant mAb, used for isotype control, was applied at concentrations about four times higher than that used for MS17-57.
Li, M., Gao, J., Feng, R., Wang, Y., Chen, X., Sun, J., ... & Lee, J. E. (2013). Generation of monoclonal antibody MS17-57 targeting secreted alkaline phosphatase ectopically expressed on the surface of gastrointestinal cancer cells. PloS one, 8(10).
IP
Figure 2 Pull-down assay for MS17-57 targets from cellular lysates of BGC823 and MKN45 cell lines.
MS17-57 mAb can pull down the targets from the cellular lysates of both BGC823 and MKN45 cell lines in direct IP.
Li, M., Gao, J., Feng, R., Wang, Y., Chen, X., Sun, J., ... & Lee, J. E. (2013). Generation of monoclonal antibody MS17-57 targeting secreted alkaline phosphatase ectopically expressed on the surface of gastrointestinal cancer cells. PloS one, 8(10).
ELISA
Figure 3 Binding of MS17-57 to purified GI cancer markers and lysates of fresh tissues and cells.
ELISA results showed that MS17-57 bound to lysates of fresh GC tissues and to lysates of GC MKN45 cells, but not to fresh lysates of adjacent noncancerous tissues from the same patients. MS17-57 bound slightly to the purified CA15-3 protein but not to proteins or lysates of PG-1, PG-2, CEA, or H. pylori. ELISA used two dose-dependent dilutions of antibodies. Normal mouse serum protein was used as a negative control.
Li, M., Gao, J., Feng, R., Wang, Y., Chen, X., Sun, J., ... & Lee, J. E. (2013). Generation of monoclonal antibody MS17-57 targeting secreted alkaline phosphatase ectopically expressed on the surface of gastrointestinal cancer cells. PloS one, 8(10).
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HeLa
Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/38764/1046_F10_4_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong membranous and cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/38765/98876_A_1_7_selected.jpg
Normal Tissue
Figure 3 Placenta
Trophoblastic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/38764/98884_A_1_7.jpg
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Specifications
- Host Species
- Mouse
- Specificity
- Human
- Clone
- MS17- 57
- Applications
- Inhib, IP, ELISA
- Related Disease
- Gastric cancer, digestive tract tumors, digestive tract tumor metastasis
Applications
- Application Notes
- This antibody has been reported for use in Inhibition, Immunoprecipitation and Enzyme-linked Immunosorbent Assay. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. should be carefully titrated in specific assays.
Target
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Adjustable to Protocol Changes
Everyone in the lab has responded in an incredibly good way. Everyone acknowledges that this antibody significantly improves the standard and reliability of our research.
Excellent Technical Assistance
The technical support team provided thorough and prompt answers to all inquiries. Their assistance has been essential to ensuring the success of our experiments.
Very little background noise
The assays revealed very little background noise, which significantly improved the experiment's signal-to-noise ratio and yielded more precise results.
Q&As
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How can I reduce my samples' proteolytic degradation?
A: To reduce deterioration, work rapidly, store samples on cold, and incorporate protease inhibitors into your lysis buffer.
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Why is the validation of antibodies important?
A: By guaranteeing that the antibody binds to the target antigen precisely and efficiently, validation upholds the validity of the experimental findings and maintains their consistency throughout several investigations.
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Is it possible to reuse primary antibodies in research?
A: Reuse is conceivable, but it can provide uneven outcomes. To guarantee consistency and repeatability in your research, it is advised to use fresh aliquots.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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See other products for "Clone MS17- 57"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-146-sdAb | Recombinant Anti-Human ALPP VHH Single Domain Antibody | FUNC, FA, IP, ChiP | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0300CL-S(P) | Anti-Human ALPP Recombinant Antibody scFv Fragment (MS17-57) | FC, ELISA, IHC, FuncS, Inhib | |
| TAB-0300CL-F(E) | Anti-Human ALPP Recombinant Antibody Fab Fragment (MS17-57) | FC, ELISA, IHC, FuncS, Inhib |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0786MZ | Mouse Anti-ALPP Recombinant Antibody (clone 9C7.29) | ELISA | Mouse IgG2a |
| MRO-0058-CN | Mouse Anti-ALPP Recombinant Antibody (clone 6-10) | WB, IF, IHC, FC | Mouse IgG2b |
| MRO-0059-CN | Rabbit Anti-ALPP Recombinant Antibody (clone CBACN-020) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-QX52 | Mouse Anti-ALPP Recombinant Antibody (clone 8A11-C11-C9) | WB | Mouse IgG1 |
| VS3-QX53 | Mouse Anti-ALPP Recombinant Antibody (clone 3C11-E7-A1) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0157 | Hi-Affi™ Rabbit Anti-ALPP Recombinant Antibody (clone DS157AB) | IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0014-YJ-S(P) | Mouse Anti-ALPP Recombinant Antibody (clone H17E2); scFv Fragment | IHC, ELISA | Mouse scFv |
| FAMAB-0244JF-S(P) | Mouse Anti-ALPP Recombinant Antibody (clone E6); scFv Fragment | ELISA, FuncS | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0014-YJ-F(E) | Mouse Anti-ALPP Recombinant Antibody (clone H17E2); Fab Fragment | IHC, ELISA | Mouse Fab |
| FAMAB-0244JF-F(E) | Mouse Anti-ALPP Recombinant Antibody (clone E6); Fab Fragment | ELISA, FuncS | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY11 | AbPlus™ Anti-ALPP Magnetic Beads (MS17- 57) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY111 | Mouse Anti-ALPP (clone H17E2) scFv-Fc Chimera | IHC, ELISA, FC | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY103 | Anti-ALPP Immunohistochemistry Kit | IHC | |
| VS-0525-XY338 | Anti-Mouse ALPP Immunohistochemistry Kit | IHC | |
| VS-0525-XY337 | Anti-Human ALPP Immunohistochemistry Kit | IHC |
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