Anti-Human ALPP Recombinant Antibody (MS17-57) (CAT#: TAB-0300CL)

Figure 1 MS17-57 inhibits BGC823 cell growth.

MS17-57 was added to BGC823 cells (8 µg/mL per well) on 96-well tissue culture plates at day 0. MS17-57 inhibited BGC823 cell growth by about 27.5% for up to 5 days. Irrelevant mAb, used for isotype control, was applied at concentrations about four times higher than that used for MS17-57.

Li, M., Gao, J., Feng, R., Wang, Y., Chen, X., Sun, J., ... & Lee, J. E. (2013). Generation of monoclonal antibody MS17-57 targeting secreted alkaline phosphatase ectopically expressed on the surface of gastrointestinal cancer cells. PloS one, 8(10).

Figure 2 Pull-down assay for MS17-57 targets from cellular lysates of BGC823 and MKN45 cell lines.

MS17-57 mAb can pull down the targets from the cellular lysates of both BGC823 and MKN45 cell lines in direct IP.

Li, M., Gao, J., Feng, R., Wang, Y., Chen, X., Sun, J., ... & Lee, J. E. (2013). Generation of monoclonal antibody MS17-57 targeting secreted alkaline phosphatase ectopically expressed on the surface of gastrointestinal cancer cells. PloS one, 8(10).

Figure 3 Binding of MS17-57 to purified GI cancer markers and lysates of fresh tissues and cells.

ELISA results showed that MS17-57 bound to lysates of fresh GC tissues and to lysates of GC MKN45 cells, but not to fresh lysates of adjacent noncancerous tissues from the same patients. MS17-57 bound slightly to the purified CA15-3 protein but not to proteins or lysates of PG-1, PG-2, CEA, or H. pylori. ELISA used two dose-dependent dilutions of antibodies. Normal mouse serum protein was used as a negative control.

Li, M., Gao, J., Feng, R., Wang, Y., Chen, X., Sun, J., ... & Lee, J. E. (2013). Generation of monoclonal antibody MS17-57 targeting secreted alkaline phosphatase ectopically expressed on the surface of gastrointestinal cancer cells. PloS one, 8(10).