Mouse Anti-ATP1B1 Recombinant Antibody (clone M17-P5-F11)

CAT#: NEUT-031CQ

This antibody is a mouse monoclonal antibody that binds specifically to Sodium/Potassium ATPase beta 1, and it can neutralize the bioactivity of Sodium/Potassium ATPase beta 1.

Gene Expression
Figure 1 Cerebral cortex Figure 2 Parathyroid gland Figure 3 Colon Figure 4 High expression in kidney Figure 5 Testis Figure 6 RNA cell line category: Cell line enhanced (OE19, RPTEC TERT1, SH-SY5Y)

Specifications

  • Immunogen
  • Purified lamb kidney beta sodium/potassium ATPase.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a
  • Species Reactivity
  • Human, Mouse, Dog, Drosophila, Pig, Rat, Sheep, Invertebrates
  • Clone
  • M17-P5-F11
  • Applications
  • Block, IF, IHC, IHC-P, IP, WB
  • Conjugate
  • Unconjugated

Product Property

  • Purification
  • Protein A purified
  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • ATP1B1; ATPase Na+/K+ transporting subunit beta 1; ATP1B; ATP1B1; ATPase Na+/K+ transporting subunit beta 1
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Breast cancer biomarkers at key points during disease progression
Effective Bioactivity Neutralization Observed
We selected this mouse monoclonal antibody specifically for its validated capability to neutralize the bioactivity of Sodium/Potassium ATPase beta 1. In our functional assays, the clone M17-P5-F11 performed exactly as described in the product specifications, effectively blocking the target function. This has been an invaluable tool for our research into osmoregulation mechanisms.
Breast cancer biomarkers at key points during disease progression
Distinct Staining In Immunohistochemistry
I utilized this antibody for Immunohistochemistry on paraffin-embedded tissue sections to visualize the distribution of the beta 1 subunit. The staining results were distinct and highly specific, allowing us to clearly observe the plasma membrane localization of the protein. It is a robust reagent that has significantly aided our structural studies of ATPase subunits.

Q&As

  1. Is this antibody conjugated to any labels or tags?

    A: This specific product is an unconjugated mouse monoclonal antibody. It is Protein A purified and provided in a liquid format. If you require a conjugated version for your specific application, you would need to label it yourself or use an appropriate secondary antibody for detection in your assays.

  2. What species does this antibody cross-react with besides mice?

    A: This recombinant antibody has been validated to react with a broad range of species. In addition to Mouse, it shows reactivity with Human, Dog, Rat, Pig, Sheep, Drosophila, and various Invertebrates. This broad cross-reactivity makes it a versatile tool for comparative research involving the ATP1B1 protein.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "ATP1B1"

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VS3-FY1657 Rabbit Anti-ATP1B1 Recombinant Antibody (clone R02-8B6) WB, IHC-P Rabbit IgG
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VS-0525-XY607 Anti-ATP1B1 Immunohistochemistry Kit IHC
CAT Product Name Application Type
VS-0525-XY608 Anti-Mouse ATP1B1 Immunohistochemistry Kit IHC
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