Hi-Affi™ Rabbit Anti-ATP6V1D Recombinant Antibody (clone DS299AB)
CAT#: MOR-0299
Recombinant Rabbit anti-ATP6V1D monoclonal antibody for WB, IHC-P, ICC/IF, IP, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57316/1001_A5_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31515/120209_B_9_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31515/120209_A_8_5.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100554-ATP6V1D
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Specifications
- Immunogen
- Synthetic peptide, corresponding to residues in Human ATP6V1D.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- Rabbit IgG
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS299AB
- Applications
- WB, IHC-P, IF, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- ATPase, H+ Transporting, Lysosomal 34kDa, V1 Subunit D; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump); V-ATPase 28 KDa Accessory Protein; Vacuolar Proton Pump Subunit D; V-ATPase Subunit D; ATP6M; VATD; H(+)-Transporting Two-Sector ATPase, Subunit M; ATPase, H+ Transporting Lysosomal, Member M; Vacuolar Proton Pump Delta Polypeptide; Vacuolar Proton-ATPase Subunit D; Vacuolar ATP Synthase Subunit D; Vacuolar Proton Pump D Subunit; Vacuolar H-ATPase Subunit D; V-ATPase D Subunit; VMA8
- Gene ID
- 51382
- UniProt ID
- Q9Y5K8
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Related Signaling Pathways
cAMP Signaling Pathway
Related Diseases
Morphine Addiction
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "ATP6V1D"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0492MZ | Mouse Anti-ATP6V1D Recombinant Antibody (clone CBLS-052) | WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC573 | AbPlus™ Anti-ATP6V1D Magnetic Beads (VS-0724-YC573) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY639 | Anti-ATP6V1D Immunohistochemistry Kit | IHC |
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