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    Recombinant Anti-human C12orf4 Intrabody [(D-Arg)9] (CAT#: IAB-B051(A))

    Recombinant anti-C12orf4 intrabody linked to (D-Arg)9 works within cells and specifically reacts with protein C12orf4, expressed in E. cili cells. It has been showed an inhibition of mast cell activation and a decrease in allergic mediator release.

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    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nuclear bodies.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37871/if_selected.jpg
    Normal Tissue
    Figure 2 IHC staining of human kidney
    Figure 2 IHC staining of human kidney
    (Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37871/ihc_selected.jpg
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Neuronal cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Neuropil
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37871/80715_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37871/80715_A_8_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37871/80715_A_8_5.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37871/80715_A_8_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37871/80715_A_5_6.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/37871/80715_A_8_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Low cell line specificity
    Figure 9 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000047621-C12orf4

    Specifications

    • Immunogen
    • human chromosome 12 open reading frame 4
    • Host Species
    • human
    • Type
    • scFv-(D-Arg)9
    • Cell Penetrating Peptide
    • (D-Arg)9
      It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
    • Species Reactivity
    • human
    • Applications
    • Used for immunoassay techniques such as: Immunofluorescence; Flow Cytometry; Western blot; Functional studies

    Product Property

    • Purity
    • >95% by HPLC
    • Storage
    • Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.

    Target

    • Alternative Names
    • C12ORF4; chromosome 12 open reading frame 4; uncharacterized protein C12orf4

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "C12orf4"

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    Please select product type
    Intrabody Products
    CAT Product Name Application Type
    IAB-B051(G) Recombinant Anti-human C12orf4 Intrabody [+36 GFP] WB, CO-IP, FuncS scFv-(+36GFP)
    IAB-B051(T) Recombinant Anti-human C12orf4 Intrabody [Tat] IF, ELISA, FuncS scFv-Tat

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