Mouse Anti-CALD1 Recombinant Antibody (clone i-DBME)
CAT#: MOB-0289CT
Recombinant Mouse monoclonal antibody to Human CALD1, expressed in mammalian cells.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8066/9_B12_1_selected.jpg
Normal Tissue
Figure 2 High expression in smooth muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8066/21805_B_6_7_val_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8066/21805_B_3_6_val_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6/2115_B_7_5.jpg
Normal Tissue
Figure 5 Smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6/2115_B_6_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ hTERT+, fHDF/TERT166, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000122786-CALD1
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Specifications
- Immunogen
- BALB/c mice were immunized with a crude extract of human uterus tissue, which served as the immunogen.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- This antibody is specific to a 150 kDa protein known as h caldesmon.
- Species Reactivity
- Human
- Clone
- i-DBME
- Applications
- IF, IHC-FoFr, IHC-Fr, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Format
- Liquid
- Concentration
- 0.2 mg/ml
- Buffer
- 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CALD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0439 | Hi-Affi™ Rabbit Anti-CALD1 Recombinant Antibody (clone DS439AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0212-CN | Rabbit Anti-CALD1 Recombinant Antibody (clone CBACN-077) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0061J | Mouse Anti-CALD1 Recombinant Antibody (ZG-0061J) | IHC-P, IF | Mouse IgG |
| VS3-WK881 | Mouse Anti-CALD1 Recombinant Antibody (VS3-WK881) | WB, IHC | Mouse IgG2b |
| VS3-WK882 | Mouse Anti-CALD1 Recombinant Antibody (clone ABT-CALD) | IHC-P, WB | Mouse IgG2b, κ |
| VS3-WK883 | Rabbit Anti-CALD1 Recombinant Antibody (VS3-WK883) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1289 | AbPlus™ Anti-CALD1 Magnetic Beads (VS-0724-YC1289) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY295 | Anti-CALD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY939 | Anti-Rat CALD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY938 | Anti-Human CALD1 Immunohistochemistry Kit | IHC |
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