Mouse Anti-CALD1 Recombinant Antibody (ZG-0061J)
CAT#: ZG-0061J
This product is a mouse antibody that recognizes CALD1.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-CALD1 Recombinant Antibody (ZG-0061J) in IHC
Immunohistochemical analysis of paraffin-embedded Liomyoma. 1. Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
IHC
Figure 2 Mouse Anti-CALD1 Recombinant Antibody (ZG-0061J) in IHC
Immunohistochemical analysis of paraffin-embedded Colon. 1. Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH8.0 was used for antigen retrieval. 3, Secondary Antibody was diluted at 1:200 (room temperature, 30min).
WB
Figure 3 Mouse Anti-CALD1 Recombinant Antibody (ZG-0061J) in WB
Western blot analysis of Caldesmon Antibody at 1:1000 dilution.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & actin filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8066/9_B12_1_selected.jpg
Normal Tissue
Figure 2 High expression in smooth muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8066/21805_B_6_7_val_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/8066/21805_B_3_6_val_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6/2115_B_7_5.jpg
Normal Tissue
Figure 5 Smooth muscle
Smooth muscle cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/6/2115_B_6_7.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ hTERT+, fHDF/TERT166, U-138 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000122786-CALD1
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Specifications
- Immunogen
- Synthesized peptide derived from human Caldesmon
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CALD1
- Species Reactivity
- Human, Mouse
- Applications
- IHC-P, IF (paraffin section)
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Immunohistochemistry-Paraffin, Immunofluorescence (paraffin section).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CALD1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0289CT | Mouse Anti-CALD1 Recombinant Antibody (clone i-DBME) | IF, IHC-FoFr, IHC-Fr, IHC-P | Mouse IgG1 |
| VS3-WK881 | Mouse Anti-CALD1 Recombinant Antibody (VS3-WK881) | WB, IHC | Mouse IgG2b |
| VS3-WK882 | Mouse Anti-CALD1 Recombinant Antibody (clone ABT-CALD) | IHC-P, WB | Mouse IgG2b, κ |
| VS3-FY181 | Recombinant Rabbit Anti-CALD1 Antibody (clone R05-3D2) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY182 | Recombinant Mouse Anti-CALD1 Antibody (clone 9B7-7G7-7A1) | IHC-P | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0439 | Hi-Affi™ Rabbit Anti-CALD1 Recombinant Antibody (clone DS439AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1289 | AbPlus™ Anti-CALD1 Magnetic Beads (VS-0724-YC1289) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY295 | Anti-CALD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY939 | Anti-Rat CALD1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY938 | Anti-Human CALD1 Immunohistochemistry Kit | IHC |
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