Recombinant Anti-human CCNT1 Intrabody [Tat] (CAT#: IAB-B032(T))
Recombinant anti-CCNT1 intrabody in fusion with HIV Tat works within cells to bind human CCNT1, expressed in 293T cells. Anti-hCyclinT1 intrabodies can be designed to block HIV-1 replication without causing cellular toxicity, and as a result, they may be useful agents for "intracellular immunization"-based gene therapy strategies for HIV-1 infection/AIDS.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4892/if_selected.jpg

(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/72012/1859_D8_31_selected.jpg

(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9016/if_selected.jpg

(Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9016/27576_A_5_8_selected.jpg

(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4892/16364_A_8_8.jpg

(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4892/16364_B_7_5.jpg

(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4892/16364_A_8_3.jpg

(Bowman's capsule Staining: Low Intensity: Moderate Quantity: <25% Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Collecting ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4892/16364_A_7_5.jpg

(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4892/16364_A_6_6.jpg

(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000129315-CCNT1
Specifications
- Immunogen
- human cyclin T1
- Host Species
- human
- Type
- scFv-Tat
- Cell Penetrating Peptide
- TAT
TAT was able to deliver different proteins, such as horseradish peroxidase and RNase A across cell membrane into the cytoplasm in different cell lines in vitro. The size range of proteins with effective delivery is from 30kDa to 120-150kDa. In one study, TAT-fused proteins are rapidly internalized by lipid raft−dependent macropinocytosis using a transducible TAT−Cre recombinase reporter assay on live cells. In another study, a TAT-fused protein was delivered into mitochondria of breast cancer cells and decreased the survival of breast cancer cells, which showed capability of TAT-fusion proteins to modulate mitochondrial function and cell survival. However, very few in vivo studies have succeeded. In one study, in vivo delivery of TAT- or penetratin-crosslinked Fab fragments yielded varied organ distributions and an overall increase in organ retention, which showed tissue localization.
- Species Reactivity
- human
- Applications
- Can be useful in applications such as: Enzyme-linked Immunosorbent Assay; Immunocytochemistry; Functional studies
Product Property
- Purity
- >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CCNT1; cyclin T1; CCNT; CYCT1; HIVE1; cyclin-T1; cyclin C-related protein; CDK9-associated C-type protein; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
- Gene ID
- 904
- UniProt ID
- O60563
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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See other products for "CCNT1"
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CAT | Product Name | Application | Type |
---|---|---|---|
IAB-B032(A) | Recombinant Anti-human CCNT1 Intrabody [(D-Arg)9] | FACS, ELISA, IF, FuncS | scFv-(D-Arg)9 |
IAB-B032(G) | Recombinant Anti-human CCNT1 Intrabody [+36 GFP] | FACS, ICC, FuncS | scFv-(+36GFP) |
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