Anti-Human CD24 Recombinant Antibody (CAT#: TAB-113CL)
Recombinant Humanized monoclonal antibody expressed in CHO binding to Human CD24. Anti-CD24 antibodiess and adjuvant combinations thereof with chemotherapeutic agents or toxins, which can be used to inhibit growth of CD24-expressing cancer cells and prevent and treat cancer.
Specific Inquiry
ELISA
Figure 1 Anti-Human CD24 Antibody (TAB-113CL) in ELISA
ELISA analysis of TAB-113CL was performed by coating with recombinant human CD24 (RPB143Hu01). Then blocked with BSA and incubated with Anti-Human CD24 Antibody (TAB-113CL) at a starting concentration of 1 ng/mL. The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human CD24 Antibody (TAB-113CL) in WB
Western blot analysis was performed by loading recombinant human CD24 (RPB143Hu01) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human CD24 Antibody (TAB-113CL) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Then probed with Goat anti-Human IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Non-Reducing Antigen (0.05 μg)
Lane 2: Reducing Antigen (0.05 μg)
Specifications
- Derivation
- Humanized
- Type
- Antibody
- Species Reactivity
- Human
- Related Disease
- Cancer
Product Property
- Purity
- >95%, by SDS-PAGE with Coomassie Brilliant Blue staining
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD24; CD24 molecule; CD24A; signal transducer CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen)
- Gene ID
- 100133941
- UniProt ID
- B6EC88
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Mouse Antibody Products
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-006LC | Mouse Anti-CD24 Recombinant Antibody (TAB-006LC) | FC, FuncS | Mouse IgG2a |
| TAB-006LC-S(P) | Mouse Anti-CD24 Recombinant Antibody; scFv Fragment (TAB-006LC-S(P)) | FC | Mouse scFv |
| TAB-006LC-F(E) | Mouse Anti-CD24 Recombinant Antibody; Fab Fragment (TAB-006LC-F(E)) | FC | Mouse Fab |
Customer Reviews and Q&As
Customer Review
Q&As
Perfect for Western Blot
I used the Anti-Human CD24 Recombinant Antibody for Western blotting, and it worked perfectly. The bands were sharp, and there was no background noise, even at a high concentration.
Efficient Cell Sorting
This antibody was perfect for sorting CD24 positive cells. The binding was specific, and the sorted cells maintained good viability. It's my go-to for cell sorting assays.
Cost-Effective and Reliable
Compared to other antibodies I've used, this one is both reliable and cost-effective. It gave consistent results across different experiments, making it an excellent investment for my research projects.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-113CL, RRID: AB_3111819)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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