Mouse Anti-CD300A Recombinant Antibody (clone 44C7)
CAT#: MOB-3424z
This product is a mouse antibody that recognizes human CD300A. It can be used in the following applications: WB, IP, IF, FCM.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line THP-1
Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10971/1944_A12_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in Kupffer cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11645/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11645/36629_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11645/36629_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11645/36629_A_6_6.jpg
Normal Tissue
Figure 6 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11645/36629_B_5_4.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11645/36629_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (HMC-1, NB-4, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167851-CD300A
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Specifications
- Immunogen
- Human CD300a Molecule
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Antibody Isotype
- IgG1
- Specificity
- Human CD300A
- Species Reactivity
- Human
- Clone
- 44C7
- Applications
- WB, IP, IF, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunoprecipitation Assay, Immunofluorescence Assay, Flow Cytometry.
Target
- Alternative Names
- CD300A; CMRF35H; Inhibitory Receptor Protein 60; IGSF12; IRC1/IRC2; CMRF35-H9; Immunoglobulin Superfamily Member 12; CMRF35-Like Molecule 8; CD300a Molecule; CMRF35H9; IRp60; NK Inhibitory Receptor; CMRF-35-H9; CLM-8
- UniProt ID
- Q9UGN4
REVIEWS AND Q&AS
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Versatile For Multiple Applications
It is rare to find an antibody that works well across so many applications. We validated clone 44C7 for Western Blot, Immunoprecipitation, and Flow Cytometry. In all cases, it recognized human CD300A effectively. This versatility allowed us to use a single reagent for different stages of our project.
Clear Immunofluorescence Signals
We used this mouse antibody for immunofluorescence assays to visualize the localization of CD300A. The images were clear with specific membrane staining patterns. The product arrived on ice packs as expected, and the quality has been consistent across the two lots we have ordered so far.
Q&As
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What applications has this clone 44C7 been reported for?
A: This Recombinant Mouse Anti-CD300A Antibody (clone 44C7) is very versatile. It has been reported for use in Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), and Flow Cytometry (FC). It specifically recognizes the Human CD300a molecule.
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Is this antibody monoclonal and what is the purity?
A: Yes, this is a mouse monoclonal antibody with the isotype IgG1. It is a recombinant antibody with a purity of greater than 95% as determined by SDS-PAGE analysis, ensuring high specificity and consistency for your research into inhibitory receptors.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "CD300A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-104LC | Rat Anti-CD300A Recombinant Antibody (TAB-104LC) | FC, Block, WB, FuncS | Rat IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-105LC | Mouse Anti-CD300A Recombinant Antibody (TAB-105LC) | FC, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-104LC-S(P) | Rat Anti-CD300A Recombinant Antibody; scFv Fragment (TAB-104LC-S(P)) | FC, WB | Rat scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-105LC-S(P) | Mouse Anti-CD300A Recombinant Antibody; scFv Fragment (TAB-105LC-S(P)) | FC, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-104LC-F(E) | Rat Anti-CD300A Recombinant Antibody; Fab Fragment (TAB-104LC-F(E)) | FC, WB | Rat Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0050MZ | Mouse Anti-CD300A Recombinant Antibody (clone NEM-261) | FC, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0551 | Hi-Affi™ Rabbit Anti-CD300A Recombinant Antibody (clone DS551AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY34 | CytoStream™ Rat Anti-CD300A Recombinant Antibody (clone 172224) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC268 | Recombinant Anti-CD300A Vesicular Antibody, EV Displayed (VS-0425-YC268) | ELISA, FC, Cell-uptake |
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