Anti-Human CD63 Recombinant Antibody (NIBR63/1) (CAT#: TAB-327LC)
Recombinant monoclonal antibody to CD63. NIBR63/1 is a mouse monoclonal antibody that can be potentially used in the treatment of rheumatoid arthritis, allergic disorders and asthma.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
FC
Figure 1 Novel anti human CD63 mAbs with specificity for the activated mast cell state.
Generation of degranulation-specific anti human mast cell Abs. Human cord blood derived mast cells (HCB Cs) were either stimulated with PMA ionomycin to induce degranulation (open graphs) or were left untreated (black), and subsequently stained with selected degranulation-specific antibodies.
FC
Figure 2 Mutant-based epitope mapping for degranulation-specific anti human mast cell CD63 mAbs.
A CD63 N 72Q single side mutation alters the binding characteristics of a degranulation- specific anti CD63 Ab. RBL cells were stably transfected either with a wild-type copy of the human CD63 gene (white), or a N172Q mutant allele (black), and subsequently stained with indicated Abs. Note a fluorescent intensity shift of mutant over wild-type cells, when surface labeled with degranulation specific anti human mast cell Ab NIBR63/1. In contrast, the staining efficiency of a commercial anti CD63 Ab (Chemicon Int. CBL553) is not affected by the N172Q mutation (left).
Inhib
Figure 3 Novel anti human CD63 mAbs selectively inhibit the degranulation of pre-activated mast cells.
Various commercial anti CD63 Abs impair human mast cell degranulation. IgE/antigen induced degranulation of human cord blood derived mast cells as followed by the enzymatic turnover of reporter enzyme β-hexosaminidase. Cells had been pre-incubated in presence (faint bars) or absence (dark bars) of indicated anti CD63 mAbs for 2.5h. Note significantly impaired degranulation efficiency in presence of the commercial mAbs #95 (Serotec MCA2142), #173 (Ancell 215-020), or #197 (MUV, aCD63-11 C9), but not for the state- specific anti human CD63 mAbs NIBR63/1-3.
Specifications
- Immunogen
- 5E5 PMA/ionomycin treated CBHMCs
- Host Species
- Mouse
- Specificity
- Human
- Clone
- NIBR63/1
- Applications
- FC, Inhib
- Related Disease
- Rheumatoid arthritis, Allergic disorders, Asthma
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry, Inhibition.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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