CytoStream™ Mouse Anti-CD63 Recombinant Antibody (clone AD1)
CAT#: VS-0225-XY64
This mouse-derived antibody targets CD63, a key protein involved in exosome-mediated cellular communication. It is specifically reactive with rat samples, making it an essential tool for rat-related research. The antibody is optimized for a range of applications, including Flow Cytometry (FC), Immunohistochemistry (IHC), and Immunoprecipitation (IP), offering researchers a reliable solution for investigating the role of CD63 in cellular processes and disease mechanisms.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line SK-MEL-30
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73506/1798_B5_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26356/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subsets of bone marrow poietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26356/ihc_selected.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26867_A_4_6.jpg
Normal Tissue
Figure 8 Bone marrow
Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10088/26880_B_6_4.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (ASC diff)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000135404-CD63
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Specifications
- Immunogen
- Rat basophilic leukemia cell line
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- CD63
- Species Reactivity
- Rat
- Clone
- AD1
- Applications
- FC, IHC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- Sodium azide
- Storage
- Store at 4°C for short-term storage and at -20°C for long-term storage. Avoid repeated freeze-thaw cycles.
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CD63"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0575 | Hi-Affi™ Rabbit Anti-CD63 Recombinant Antibody (clone DS575AB) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0071-YJ-S(P) | Human Anti-CD63 Recombinant Antibody (clone Hu33-11A); scFv Fragment | FC, ELISA, IHC | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0071-YJ-F(E) | Human Anti-CD63 Recombinant Antibody (clone Hu33-11A); Fab Fragment | FC, ELISA, IHC | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-1062F | Mouse Anti-CD63 Recombinant Antibody (clone NKI-Beteb) | IHC-Fr, IHC-P | Mouse IgG2b |
| ZG-1063F | Mouse Anti-CD63 Recombinant Antibody (clone NKI-C3) | ELISA, IF, IHC-Fr, IHC-P | Mouse IgG1 |
| VS3-XY388 | Mouse Anti-CD63 Recombinant Antibody, FITC (clone 4AH5C6) | FC, IF | Mouse IgG1 |
| VS3-XY389 | Mouse Anti-CD63 Recombinant Antibody, PE (clone 4AH5C6) | FC, IF | Mouse IgG1 |
| VS3-XY390 | Mouse Anti-CD63 Recombinant Antibody (clone 4AH5C6) | FC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY369 | Anti-CD63 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1207 | Anti-Mouse CD63 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1208 | Anti-Rat CD63 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC180 | Recombinant Anti-CD63 Vesicular Antibody, EV Displayed (VS-0425-YC180) | ELISA, FC, Inhib, Cell-uptake |
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