Hi-Affi™ Rabbit Anti-CD83 Recombinant Antibody (clone DS586AB)
CAT#: MOR-0586
Recombinant Rabbit anti-CD83 monoclonal antibody for WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73595/1806_G9_2_selected.jpg
Normal Tissue
Figure 2 Tonsil
High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/41454/ihc_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2509/6585_A_5_6.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (Daudi, HDLM-2, U-698)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112149-CD83
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Specifications
- Immunogen
- Synthetic peptide corresponding to Human CD83.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human CD83
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS586AB
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Superior Western Blot Results
This Hi-Affi recombinant rabbit antibody (clone DS586AB) gave us superior results in Western Blot compared to traditional mouse antibodies. It specifically detected CD83 in our dendritic cell lysates with a very clean background. The high affinity is evident in the strong signal we obtained.
Reliable Marker for Dendritic Cells
We used this antibody to identify activated B lymphocytes and dendritic cells in our research. The recombinant technology ensures batch-to-batch consistency. It binds specifically to the CD83 antigen, helping us study its role in antigen presentation and immune regulation processes.
Q&As
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What cell types express the CD83 antigen?
A: CD83 is a member of the immunoglobulin superfamily and is a well-known marker for activated dendritic cells and activated B lymphocytes. This antibody specifically binds to Human CD83 and helps researchers identify and study the maturation state of these immune cells.
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What is the advantage of using a rabbit monoclonal antibody?
A: Rabbit monoclonal antibodies, like this Hi-Affi™ Recombinant antibody, typically offer higher affinity and specificity compared to mouse antibodies. They recognize a broader range of epitopes and are excellent for applications like Western Blot where high sensitivity and low background are required.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| TAB-355LC | Armenian hamster Anti-CD83 Recombinant Antibody (TAB-355LC) | ELISA, FC, FuncS | Armenian hamster IgG |
| CAT | Product Name | Application | Type |
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| TAB-356LC | Armenian hamster Anti-CD83 Recombinant Antibody (TAB-356LC) | ELISA, FC, FuncS | Armenian hamster IgG |
| CAT | Product Name | Application | Type |
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| TAB-357LC | Armenian hamster Anti-CD83 Recombinant Antibody (TAB-357LC) | ELISA, FC, FuncS | Armenian hamster IgG |
| CAT | Product Name | Application | Type |
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| TAB-355LC-S(P) | Armenian hamster Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-355LC-S(P)) | ELISA, FC | Armenian hamster scFv |
| CAT | Product Name | Application | Type |
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| TAB-356LC-S(P) | Armenian hamster Anti-CD83 Recombinant Antibody; scFv Fragment (TAB-356LC-S(P)) | ELISA, FC | Armenian hamster scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| HPAB-0128-LSX | Human Anti-CD83 Recombinant Antibody (HPAB-0128-LSX) | ELISA, FC | Human IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0129-LSX | Human Anti-CD83 Recombinant Antibody (HPAB-0129-LSX) | ELISA, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0130-LSX | Human Anti-CD83 Recombinant Antibody (HPAB-0130-LSX) | ELISA, FC | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0128-LSX-S(P) | Hamster Anti-CD83 Recombinant Antibody; scFv Fragment (HPAB-0128-LSX-S(P)) | ELISA, FC, FC | Hamster scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0129-LSX-S(P) | Hamster Anti-CD83 Recombinant Antibody; scFv Fragment (HPAB-0129-LSX-S(P)) | ELISA, FC, FC | Hamster scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0130-LSX-S(P) | Hamster Anti-CD83 Recombinant Antibody; scFv Fragment (HPAB-0130-LSX-S(P)) | ELISA, FC, FC | Hamster scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0128-LSX-F(E) | Human Anti-CD83 Recombinant Antibody; Fab Fragment (HPAB-0128-LSX-F(E)) | ELISA, FC | Chimeric (hamster/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0129-LSX-F(E) | Human Anti-CD83 Recombinant Antibody; Fab Fragment (HPAB-0129-LSX-F(E)) | ELISA, FC | Chimeric (hamster/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0130-LSX-F(E) | Human Anti-CD83 Recombinant Antibody; Fab Fragment (HPAB-0130-LSX-F(E)) | ELISA, FC | Chimeric (hamster/human) Fab |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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