Hi-Affi™ Rabbit Anti-CDKN2A Recombinant Antibody (clone DS642AB)
CAT#: MOR-0642
Recombinant Rabbit anti-CDKN2A monoclonal antibody for FC, ICC/IF, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47838/979_F9_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/93/ihc_selected.jpg
Normal Tissue
Figure 3 Tonsil
High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_4_8_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows strong positivity in a subset of cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18232/ihc_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_8.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/445/2553_A_8_5.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (CAPAN-2, HEK93, HeLa, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147889-CDKN2A
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Specifications
- Immunogen
- Recombinant full length Human CDKN2A/p16INK4a tagged.
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human CDKN2A
- Species Reactivity
- Human
- Clone
- DS642AB
- Applications
- FC, ICC, IF, WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Tissue culture supernatant
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Cyclin-Dependent Kinase Inhibitor 2A; Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4); Cyclin-Dependent Kinase 4 Inhibitor A; Multiple Tumor Suppressor 1; Alternative Reading Frame; P16-INK4A; P16INK4A; P14ARF; CDK4I; CDKN2; MTS-1; MTS1; MLM; ARF; Cell Cycle Negative Regulator Beta; CDK4 Inhibitor P16-INK4; P16-INK4; P16INK4; P19ARF; INK4A; CMM2; INK4; TP16; P19; P14; P16
- Gene ID
- 1029
- UniProt ID
- Q8N726
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1779z | Mouse Anti-CDKN2A Recombinant Antibody (clone 30C1) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| MOB-2201MZ | Recombinant Mouse Anti-Human CDKN2A Antibody (clone 25Q03 (tbnf bt EDT-350)) | ICC, IF, IP, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0116MZ | Chicken Anti-CDKN2A Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| HPAB-0877LY-S(P) | Mouse Anti-CDKN2A Recombinant Antibody (clone CBL015); scfv Fragment | IHC, WB | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0877LY | Mouse Anti-CDKN2A Recombinant Antibody (clone CBL015) | IHC, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0877LY-F(E) | Mouse Anti-CDKN2A Recombinant Antibody (clone CBL015); Fab Fragment | IHC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1141-CN | Rabbit Anti-CDKN2A Recombinant Antibody (clone CBACN-415) | WB, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1769-CN | Rabbit Anti-CDKN2A Polyclonal Antibody (MRO-1769-CN) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0037-YC-S(P) | Mouse Anti-CDKN2A Recombinant Antibody (clone P1); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0037-YC-F(E) | Mouse Anti-CDKN2A Recombinant Antibody (clone P1); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1355 | AbPlus™ Anti-CDKN2A Magnetic Beads (VS-0724-YC1355) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY414 | Anti-CDKN2A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1341 | Anti-Rat CDKN2A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY1340 | Anti-Human CDKN2A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC16 | Anti-CDKN2A Antibody Prodrug, Protease Activated (VS-1025-YC16) | ISZ, Cyt, FuncS |
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