What antigen does this antibody recognize?

This recombinant Mouse antibody recognizes NCA-90, which is also known as CEACAM6. The immunogen used was partially purified carcinoembryonic antigen (CEA) derived from liver metastases of human colonic adenocarcinoma.

Mouse Anti-CEACAM6 Recombinant Antibody (TAB-478MZ)

CAT#: TAB-478MZ

This product is a recombinant Mouse antibody that recognizes NCA-90. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression

Normal Tissue Figure 1 IHC staining of human duodenum

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in a subset of leukocytes.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11041/ihc_selected.jpg

Normal Tissue Figure 2 High expression in lung.

Macrophages Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11041/27139_A_3_4.jpg

Normal Tissue Figure 3 Colon

Enterocytes - Microvilli Staining: Medium Intensity: Moderate Mucosal lymphoid cells Staining: Medium Intensity: Strong

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11041/27139_A_9_3.jpg

Normal Tissue Figure 4 Lymph node

Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11041/27139_A_8_8.jpg

RNA Expression Figure 5 RNA cell line category: Cell line enriched (OE19)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000086548-CEACAM6

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Specifications

Immunogen
Partially purified carcinoembryonic antigen (CEA) derived from liver metastases of human colonic adenocarcinoma

Host Species
Mouse

Type
Mouse IgG

Specificity
Human NCA-90

Species Reactivity
Human

Applications
Enzyme-linked Immunosorbent Assay

Related Disease
Chronic myelocytic leukemia (CML)

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
NCA; CEAL; CD66c

Gene ID
4680

UniProt ID
P40199

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Breast cancer biomarkers at key points during disease progression

Specific Detection of NCA-90

This recombinant mouse antibody specifically recognizes NCA-90 (CEACAM6), as confirmed by our ELISA results. It showed no cross-reactivity issues and provided clean data. It is a reliable reagent for our studies on cell adhesion molecules belonging to the CEA family.

High Purity Mouse Antibody

We were impressed with the quality of TAB-478MZ. The antibody was purified by affinity chromatography and showed greater than 95% purity on SDS-PAGE. Its consistent performance in detecting Human CEACAM6 has made it a standard reagent for our tumor marker research and immunoassay development.

Q&As

What antigen does this antibody recognize?

A: This recombinant Mouse antibody recognizes NCA-90, which is also known as CEACAM6. The immunogen used was partially purified carcinoembryonic antigen (CEA) derived from liver metastases of human colonic adenocarcinoma.
Is this antibody monoclonal or polyclonal?

A: This is a recombinant Mouse monoclonal antibody (IgG). It is expressed in mammalian cells with chemically defined culture media and purified by affinity chromatography to ensure high specificity for CEACAM6/NCA-90.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-478MZ.pdf

MSDS

MSDS - TAB-478MZ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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