Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone TH138DS)
CAT#: MOR-4625
Recombinant Rabbit anti-CXCL8 monoclonal antibody for WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/1010_F11_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in non germinal center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line GAMG
Immunofluorescent staining of human cell line GAMG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/2058_E8_1_blue_red_green.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57179/131156_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (BJ hTERT+, GAMG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000169429-CXCL8
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Specifications
- Immunogen
- Recombinant protein corresponding to amino acids 28-99 of human Interleukin 8
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CXCL8
- Species Reactivity
- Human
- Clone
- TH138DS
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported/tested for use in Western Blot (2-3 µg/ml).
Target
- Alternative Names
- C-X-C Motif Chemokine Ligand 8; Chemokine (C-X-C Motif) Ligand 8; Monocyte-Derived Neutrophil Chemotactic Factor; Monocyte-Derived Neutrophil-Activating Peptide; Granulocyte Chemotactic Protein 1; Beta Endothelial Cell-Derived Neutrophil Activating Peptide; Lung Giant Cell Carcinoma-Derived Chemotactic Protein; Lymphocyte Derived Neutrophil Activating Peptide; Alveolar Macrophage Chemotactic Factor I; Tumor Necrosis Factor-Induced Gene 1; Neutrophil-Activating Peptide 1; T-Cell Chemotactic Factor; Interleukin 8; Emoctakin; GCP-1; MDNCF; MONAP; NAP-1; IL8; Small Inducible Cytokine Subfamily B, Member 8; Beta-Thromboglobulin-Like Protein; Neutrophil-Activating Protein 1; C-X-C Motif Chemokine 8; Protein 3-10C; LYNAP; NAP1; GCP1; LECT; LUCT; IL-8; NAF
- Gene ID
- 3576
- UniProt ID
- P10145
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| MOR-4624 | Hi-Affi™ Rabbit Anti-CXCL8 Recombinant Antibody (clone TH137DS) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1973-CN | Rabbit Anti-CXCL8 Polyclonal Antibody (MRO-1973-CN) | ELISA, IF, IHC | Rabbit IgG |
| MRO-1974-CN | Rabbit Anti-CXCL8 Polyclonal Antibody (MRO-1974-CN) | WB, IF, FC | Rabbit IgG |
| HPAB-N0059-YC | Human Anti-CXCL8 Recombinant Antibody (HPAB-N0059-YC) | FuncS | Human IgG |
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| HPAB-1311WJ | Human Anti-CXCL8 Recombinant Antibody (clone M2-11) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0059-YC-S(P) | Human Anti-CXCL8 Recombinant Antibody; scFv Fragment (HPAB-N0059-YC-S(P)) | FuncS | Human scFv |
| HPAB-1682-FY-F(E) | Human Anti-CXCL8 Recombinant Antibody; Fab Fragment (HPAB-1682-FY-F(E)) | ELISA | Human Fab |
| HPAB-1683-FY-F(E) | Human Anti-CXCL8 Recombinant Antibody; Fab Fragment (HPAB-1683-FY-F(E)) | ELISA | Human Fab |
| HPAB-1684-FY-F(E) | Human Anti-CXCL8 Recombinant Antibody; Fab Fragment (HPAB-1684-FY-F(E)) | ELISA | Human Fab |
| HPAB-1544WJ-S(P) | Human Anti-CXCL8 Recombinant Antibody; scFv Fragment (clone K2.2.1) | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1501-FY-S(P) | Mouse Anti-CXCL8 Recombinant Antibody (clone 5D2.2D10); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-1502-FY-S(P) | Mouse Anti-CXCL8 Recombinant Antibody (clone 1A1.4C6); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-1503-FY-S(P) | Mouse Anti-CXCL8 Recombinant Antibody (clone 1L351.17); Fab Fragment | ELISA, WB | Mouse Fab |
| HPAB-1310WJ-F(E) | Human Anti-CXCL8 Recombinant Antibody (clone M1-10); Fab Fragment | ELISA | Human Fab |
| HPAB-1311WJ-F(E) | Human Anti-CXCL8 Recombinant Antibody (clone M2-11); Fab Fragment | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY593 | Anti-CXCL8 Immunohistochemistry Kit | IHC |
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