Hi-Affi™ Recombinant Rabbit Anti-EIF-2ALPHA Monoclonal Antibody (DS1116AB)
CAT#: MOR-1116
Recombinant Rabbit anti-EIF-2ALPHA monoclonal antibody for ICC/IF, WB, IHC-P, ICC, Dot, IHC-Fr, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/1215_G4_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11663/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11663/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_8_5.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_6_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134001-EIF2S1
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Specifications
- Immunogen
- A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human EIF2S1.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Fruit fly
- Clone
- DS1116AB
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Western Blot; Immunohistochemistry (Paraffin); Immunocytochemistry; Dot; Immunohistochemistry-Fr
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Superior Western Blot Sensitivity
The Hi-Affi™ recombinant rabbit antibody for EIF-2ALPHA gave us superior results in Western Blot compared to traditional hybridoma antibodies. The bands were sharp, and it detected the protein in various cell lysates with very little background. A great tool for translation initiation research.
Perfect For Immunofluorescence Staining
We used this monoclonal antibody for Immunocytochemistry to visualize EIF2S1 localization. The staining pattern was precise, and the recombinant nature of the antibody ensures we get the same performance with every new lot. It is highly recommended for protein synthesis studies.
Q&As
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Is this a recombinant antibody?
A: Yes, this is a Hi-Affi™ Recombinant Rabbit Monoclonal Antibody. It is developed using recombinant technology, likely from a phage display library, which avoids the use of traditional ascitic fluid and ensures greater specificity, sensitivity, and lot-to-lot consistency.
-
What applications are recommended for this antibody?
A: We recommend this antibody for a wide range of applications, including Western Blot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunohistochemistry (Paraffin and Frozen), and Dot Blot. It is a versatile tool for producing superior results.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MOR-1116, RRID: AB_3714018)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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