Mouse Anti-NHP EIF2S1 Recombinant Antibody (clone 5A5)
CAT#: VS-1024-XY181
The antibody of mouse origin is designed to specifically recognize EIF2S1, demonstrating reactivity with human, non-human primate, and rat samples. The immunogen consists of the full-length human EIF2S1 protein, ensuring high-fidelity binding. Suitable for applications in Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), this antibody is a versatile tool for probing EIF2S1 expression, offering robust performance across multiple research platforms.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/1215_G4_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11663/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11663/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_8_5.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_6_5.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134001-EIF2S1
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Specifications
- Immunogen
- Full length human EIF2S1 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- EIF2S1
- Species Reactivity
- Human, Non-human primate, Rat
- Clone
- 5A5
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:20-1:200, IF: 1:20-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "Clone 5A5"
- CAT
- Product Name
See other products for "EIF2S1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3745z | Mouse Anti-EIF2S1 Recombinant Antibody (clone 26H8) | WB, ELISA, ICC, IF | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2291CT | Recombinant Mouse anti-Human EIF2S1 Monoclonal antibody (5A5) | ICC, IF, WB, IHC-P |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1116 | Hi-Affi™ Recombinant Rabbit Anti-EIF-2ALPHA Monoclonal Antibody (DS1116AB) | ICC, IF, WB, IHC-P, ICC, Dot, IHC-Fr | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1120 | Hi-Affi™ Rabbit Anti-EIF2S1 Recombinant Antibody (clone DS1120AB) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0284J | Mouse Anti-EIF2S1 Recombinant Antibody (ZG-0284J) | WB, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0647J | Rabbit Anti-EIF2S1 Recombinant Antibody (clone 1C6) | ELISA, WB, IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX358 | Mouse Anti-EIF2S1 Recombinant Antibody (clone 2E4-F5-E7) | WB, IF, IP | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2212 | Anti-EIF2S1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2213 | Anti-Mouse EIF2S1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2214 | Anti-Rat EIF2S1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL33 | Rabbit Anti-eIF-2a (phospho S51) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL119 | Rabbit Anti-eIF2 alpha (phospho S51) Monoclonal Antibody | Phospho Antibodies for Cell Signaling Research |
| CAT | Product Name | Application | Type |
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| VS-0126-XL2287 | Rabbit Anti-eIF-2a (phospho S52) Antibody | Phospho Antibodies for Cell Signaling Research |
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