Mouse Anti-NHP EIF2S1 Recombinant Antibody (clone 5A5) (CAT#: VS-1024-XY181)
The antibody of mouse origin is designed to specifically recognize EIF2S1, demonstrating reactivity with human, non-human primate, and rat samples. The immunogen consists of the full-length human EIF2S1 protein, ensuring high-fidelity binding. Suitable for applications in Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), this antibody is a versatile tool for probing EIF2S1 expression, offering robust performance across multiple research platforms.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/1215_G4_3_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/1215_G4_3_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11663/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11663/if_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11663/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows distinct cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/11663/ihc_selected.jpg
Figure 4 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_8_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_8_8.jpg
Figure 5 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_9_3.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_8_5.jpg
Figure 7 Urinary bladder
(Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_6_5.jpg
(Urothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64885/144577_A_6_5.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134001-EIF2S1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134001-EIF2S1
Specifications
- Immunogen
- Full length human EIF2S1 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- EIF2S1
- Species Reactivity
- Human, Non-human primate, Rat
- Clone
- 5A5
- Applications
- WB, IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:20-1:200, IF: 1:20-1:200
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3745z | Mouse Anti-EIF2S1 Recombinant Antibody (clone 26H8) | WB, ELISA, ICC, IF | Mouse IgG1, κ |
| MOB-2291CT | Recombinant Mouse anti-Human EIF2S1 Monoclonal antibody (5A5) | ICC/IF, WB, IHC-P | |
| ZG-0284J | Mouse Anti-EIF2S1 Recombinant Antibody (ZG-0284J) | WB, ICC | Mouse IgG |
| ZG-0647J | Rabbit Anti-EIF2S1 Recombinant Antibody (clone 1C6) | ELISA, WB, IHC, IF | Rabbit IgG |
| VS3-WK2254 | Rabbit Anti-EIF2S1 (pS51) Recombinant Antibody (VS3-WK2254) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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