What is the host species for this antibody?

This is a recombinant Rabbit antibody. It was expressed in mammalian cells using chemically defined culture media and purified by affinity chromatography. It recognizes human Fas ligand (FASLG/CD95L).

What part of the protein was used as the immunogen?

The antibody was raised against a synthetic N-terminal peptide encoding the first 21 amino acids of human CD95L. This specific sequence (MQQPFNYPYPQIYWVDSSASS) allows for targeted detection of the Fas ligand protein.

Rabbit Anti-FASLG Recombinant Antibody (TAB-026WM)

CAT#: TAB-026WM

This product is a recombinant Rabbit antibody that recognizes Fas ligand. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

Gene Expression

Normal Tissue Figure 1 IHC staining of human lymph node

Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in subsets of cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/ihc_selected.jpg

Normal Tissue Figure 2 Cerebral cortex

Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_B_8_5.jpg

Normal Tissue Figure 3 Kidney

Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_A_8_5.jpg

Normal Tissue Figure 4 Testis

Cells in seminiferous ducts Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_A_6_6.jpg

Normal Tissue Figure 5 Bone marrow

Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_B_5_4.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54959/134178_A_8_8.jpg

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Specifications

Immunogen
A synthetic N-terminal peptide, encoding the first 21aa of human CD95L (CD95L_NT 1-21: MQQPFNYPYPQIYWVDSSASS)

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human FASLG

Species Reactivity
Human

Applications
ELISA, IHC

Related Disease
Pemphigus

Product Property

Purity
>95% as determined by SDS-PAGE

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
FASLG; Fas ligand (TNF superfamily, member 6); APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1; tumor necrosis factor ligand superfamily member 6; CD95 ligand; fas antigen ligand; apoptosis antigen ligand; apoptosis (APO-1) antigen ligand 1

Gene ID
356

UniProt ID
P48023

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Breast cancer biomarkers at key points during disease progression

Reliable IHC Staining Results

We applied this Rabbit Anti-FASLG antibody to tissue sections to study apoptosis signaling. It specifically recognized the Fas ligand and provided clear staining in the expected regions. The affinity purification seems to have removed any non-specific binders effectively.

High Specificity For Fas Ligand

This recombinant antibody showed>95% purity on SDS-PAGE, which gave us confidence in its quality. We used it in ELISA to quantify CD95L levels in cell culture supernatants, and the results were consistent and reproducible across multiple experimental runs.

Q&As

What is the host species for this antibody?

A: This is a recombinant Rabbit antibody. It was expressed in mammalian cells using chemically defined culture media and purified by affinity chromatography. It recognizes human Fas ligand (FASLG/CD95L).
What part of the protein was used as the immunogen?

A: The antibody was raised against a synthetic N-terminal peptide encoding the first 21 amino acids of human CD95L. This specific sequence (MQQPFNYPYPQIYWVDSSASS) allows for targeted detection of the Fas ligand protein.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - TAB-026WM.pdf

MSDS

MSDS - TAB-026WM.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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