Hi-Affi™ Rabbit Anti-FOXP3 Recombinant Antibody (clone TH89DS) (CAT#: MOR-4577)
Recombinant Rabbit anti-FOXP3 monoclonal antibody for WB, IHC-P, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45943/if_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45943/if_selected.jpg
Figure 2 High expression in lymph node.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8_val_selected.jpg
Figure 3 Low expression in parathyroid gland.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_1_8_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_1_8_val_selected.jpg
Figure 4 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_4_6.jpg
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_4_6.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, MCF7, SiHa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000049768-FOXP3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000049768-FOXP3
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FOXP3
- Species Reactivity
- Human, Mouse
- Clone
- TH89DS
- Applications
- WB, IHC-P, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.09% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Flow Cytometry (3-5 µg per 1 per 10^6 cells), Immunohistochemistry-Paraffin (1:10-1:100) and Western Blot (1-3 µg/ml).
Target
- Alternative Names
- Forkhead Box P3; Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked; Scurfin; IPEX; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; FOXP3delta7; DIETER; AIID; PIDX; XPID; JM2
- Gene ID
- 50943
- UniProt ID
- Q9BZS1
Related Resources
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1071MZ | Recombinant Mouse Anti-Human FOXP3 Antibody (clone 347B/F8) | FC, IHC-Fr IHC-P, WB | Mouse antibody |
| MRO-0606-CN | Rabbit Anti-FOXP3 Recombinant Antibody (clone JF0898) | IF, FC | Rabbit IgG |
| MRO-0607-CN | Rabbit Anti-FOXP3 Recombinant Antibody (clone JF0926) | WB, FC | Rabbit IgG |
| VS7-HM970 | Mouse Anti-FOXP3 Recombinant Antibody (clone 4F12A2) | IHC, FCM | Mouse IgG1 |
| VS7-HM971 | Mouse Anti-FOXP3 Recombinant Antibody (clone 4F12F1) | FCM | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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