Mouse Anti-NHP FOXP3 Recombinant Antibody (clone SPM579) (CAT#: VS-1024-XY207)
Designed to target FOXP3 with high specificity, this mouse monoclonal antibody demonstrates reactivity across human, mouse, and non-human primate samples. The immunogen, a full-length recombinant human FOXP3 protein, ensures precise antigen recognition. Suitable for use in Western blot (WB), immunohistochemistry (IHC), and flow cytometry (FC), this antibody is a versatile tool for investigating FOXP3 expression, offering robust and reliable performance in various experimental settings.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45943/if_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45943/if_selected.jpg
Figure 2 High expression in lymph node.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8_val_selected.jpg
Figure 3 Low expression in parathyroid gland.
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_1_8_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_1_8_val_selected.jpg
Figure 4 Testis
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_4_6.jpg
(Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_4_6.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, MCF7, SiHa)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000049768-FOXP3
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000049768-FOXP3
Specifications
- Immunogen
- Full-length human FOXP3 protein
- Host Species
- Mouse
- Type
- Mouse IgG1, kappa
- Specificity
- FOXP3
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- SPM579
- Applications
- WB, IHC, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.05% BSA
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1-2 µg/mL, IHC: 1-2 µg/mL, FC: 1-2 µg/106 cells
Target
- Alternative Names
- Forkhead Box P3; Immune Dysregulation; Polyendocrinopathy; Enteropathy; X-Linked; Scurfin; IPEX; Immunodeficiency; Polyendocrinopathy; Enteropathy; X-Linked; Forkhead Box Protein P3; FOXP3delta7;
- Gene ID
- 50943
- UniProt ID
- Q9BZS1
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1348 | Hi-Affi™ Rabbit Anti-FOXP3 Recombinant Antibody (clone DS1348AB) | IHC-P | Rabbit IgG |
| MOR-4577 | Hi-Affi™ Rabbit Anti-FOXP3 Recombinant Antibody (clone TH89DS) | WB, IHC-P, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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