CytoStream™ Rabbit Anti-FOXP3 Recombinant Antibody (VS13-YC423)
CAT#: VS13-YC423
The CytoStream™ Rabbit Anti-FOXP3 Recombinant Antibody is specific for human FOXP3. FOXP3, a regulatory forkhead protein, causes X-linked IPEX syndrome when defective, affecting autoimmunity through its involvement in immune system maturation and differentiation. The anti-FOXP3 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/45943/if_selected.jpg
Normal Tissue
Figure 2 High expression in lymph node.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8_val_selected.jpg
Normal Tissue
Figure 3 Low expression in parathyroid gland.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_1_8_val_selected.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_4_6.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/26301/58210_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HDLM-2, MCF7, SiHa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000049768-FOXP3
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Specifications
- Immunogen
- Recombinant human FOXP3, aa 250-431.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human FOXP3
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/100
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Forkhead Box P3; Immune Dysregulation, Polyendocrinopathy, Enteropathy, X-Linked; Scurfin; IPEX; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; Forkhead Box Protein P3; FOXP3delta7;
- Gene ID
- 50943
- UniProt ID
- Q9BZS1
- Long Name
- Forkhead Box P3
- Cellular Localization
- Nucleus; Cytoplasm
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Related Diseases
Inflammatory Bowel Diseases
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "FOXP3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1348 | Hi-Affi™ Rabbit Anti-FOXP3 Recombinant Antibody (clone DS1348AB) | IHC-P | Rabbit IgG |
| MOR-4577 | Hi-Affi™ Rabbit Anti-FOXP3 Recombinant Antibody (clone TH89DS) | WB, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0274-YC-S(P) | Human Anti-FOXP3 Recombinant Antibody; scFv Fragment (HPAB-M0274-YC-S(P)) | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0274-YC-F(E) | Human Anti-FOXP3 Recombinant Antibody; Fab Fragment (HPAB-M0274-YC-F(E)) | FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0362J | Mouse Anti-FOXP3 Recombinant Antibody (ZG-0362J) | WB, ELISA | Mouse IgG |
| ZG-0363J | Mouse Anti-FOXP3 Recombinant Antibody (ZG-0363J) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0364J | Mouse Anti-FOXP3 Recombinant Antibody (ZG-0364J) | WB, ICC | Mouse IgG |
| VS4-WK183 | Mouse Anti-FOXP3 Recombinant Antibody (VS4-WK183) | ELISA, WB | Mouse IgG1 |
| VS-0923-FY61 | Recombinant Mouse Anti-FOXP3 Antibody (VS-0923-FY61) | ELISA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC757 | AbPlus™ Anti-FOXP3 Magnetic Beads (VS-0724-YC757) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY207 | Mouse Anti-NHP FOXP3 Recombinant Antibody (clone SPM579) | WB, IHC, FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY862 | Anti-FOXP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2643 | Anti-Mouse FOXP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2642 | Anti-Human FOXP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2644 | Anti-Monkey FOXP3 Immunohistochemistry Kit | IHC |
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