Mouse Anti-Fluorescein Recombinant Antibody (clone mAb 4-4-20) (CAT#: PABL-475)

Figure 1 Solid phase ELISA analysis of Fv 4-4-20.

A, direct binding of IgG, SCA, or Fv 4-4-20 (10 μg/ml starting concentration) to fluorescein-BSA-coated wells. Protein was detected with mAb 3A5-1 (10 μg/ml) and horseradish peroxidase-anti-hamster IgG. B, 4-4-20 proteins (10Graphic to 10GraphicM) were used to inhibit anti-metatype antibodies (10 ng/ml) from binding to wells coated with affinity-labeled Fab 4-4-20. C, 4-4-20 proteins (10Graphic to 10GraphicM) were used to inhibit horseradish peroxidase-IgG 4-4-20 (10 ng/ml) from binding to wells coated with anti-idiotype antibodies. Individual points represent mean values of triplicate trials with standard deviations (error bars). Points lacking error bars indicate standard deviations smaller than symbol.

Mallender, W. D., Carrero, J., & Voss, E. W. (1996). Comparative Properties of the Single Chain Antibody and Fv Derivatives of mAb 4-4-20 RELATIONSHIP BETWEEN INTERDOMAIN INTERACTIONS AND THE HIGH AFFINITY FOR FLUORESCEIN LIGAND. Journal of Biological Chemistry, 271(10), 5338-5346.