Mouse Anti-Fluorescein Recombinant Antibody (clone mAb 4-4-20) (CAT#: PABL-475)
Recombinant Mouse Antibody (mAb 4-4-20) is capable of binding to Fluorescein, expressed in Chinese Hamster Ovary cells (CHO). This antibody binds fluorescein with an association constant of 3.4 x 10" Mpl in aqueous solution.
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Figure 1 Solid phase ELISA analysis of Fv 4-4-20.
A, direct binding of IgG, SCA, or Fv 4-4-20 (10 μg/ml starting concentration) to fluorescein-BSA-coated wells. Protein was detected with mAb 3A5-1 (10 μg/ml) and horseradish peroxidase-anti-hamster IgG. B, 4-4-20 proteins (10Graphic to 10GraphicM) were used to inhibit anti-metatype antibodies (10 ng/ml) from binding to wells coated with affinity-labeled Fab 4-4-20. C, 4-4-20 proteins (10Graphic to 10GraphicM) were used to inhibit horseradish peroxidase-IgG 4-4-20 (10 ng/ml) from binding to wells coated with anti-idiotype antibodies. Individual points represent mean values of triplicate trials with standard deviations (error bars). Points lacking error bars indicate standard deviations smaller than symbol.
Mallender, W. D., Carrero, J., & Voss, E. W. (1996). Comparative Properties of the Single Chain Antibody and Fv Derivatives of mAb 4-4-20 RELATIONSHIP BETWEEN INTERDOMAIN INTERACTIONS AND THE HIGH AFFINITY FOR FLUORESCEIN LIGAND. Journal of Biological Chemistry, 271(10), 5338-5346.
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Clone
- mAb 4-4-20
- Applications
- Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Fluorescein
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-046 | Human Anti-Fluorescein Recombinant Antibody (clone 4M5.3) | FC | Human IgG |
| PABW-046 | Mouse Anti-Fluorescein Recombinant Antibody (clone 9-40) | WB, IF, FuncS | Mouse IgG |
| PABC-047 | Human Anti-Fluorescein Recombinant Antibody (clone E2) | WB, FuncS | Human IgG |
| FAMAB-0113YC | Chicken Anti-Fluorescein Recombinant Antibody (clone 84/85-s-3) | ELISA | Chicken IgG |
| FAMAB-0114YC | Chicken Anti-Fluorescein Recombinant Antibody (clone 84/85-F-8) | ELISA | Chicken IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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