Anti-GPA33 immunotoxin (scFv)-α-sar (CAT#: AGTO-G034F)
This immunotoxin was achieved by conjugating/fusing the Anti-GPA33 scFv to α-sarcin. It was expressed in CHO and purified with affinity chromatography. The immunotoxin selectively binds to GPA33 tumor antigen expressing cells. Once bind, alpha-Sarcin enters into the cells by receptor-mediated endocytosis and interacts with a single bond in the targeted ribosome and breaks it, causing the ribosome to be inactivated. It was designed for treatment of colon cancer.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 High expression in colon
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/ihc_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/ihc_selected.jpg
Figure 2 Low expression in liver
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/43100_A_7_4_val_selected.jpg
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/43100_A_7_4_val_selected.jpg
Figure 3 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/43100_A_9_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/43100_A_9_3.jpg
Figure 4 Appendix
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/43100_A_2_2.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18858/43100_A_2_2.jpg
Figure 5 RNA cell line category: Cell line enriched (OE19)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143167-GPA33
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000143167-GPA33
Specifications
- Host Species
- Mouse
- Species Reactivity
- Human
- Molecule Class
- scFv-α-sar
- Toxin Description
- Alpha-Sarcin is a ribosome-inactivating protein produced in the mold Aspergillus giganteus. Specifically, alpha-sarcin cleaves the a portion of ribosomal RNA that forms the small ribosomal substrate. The high specificity of alpha-sarcin and its efficiency of cleavage are point of study and also account for this protein's very high toxicity level.
- Applications
- This product is suitable for use in cytotoxicity assay, functional assay and immunotherapy.
- Related Disease
- colon cancer
Target
- Alternative Names
- GPA33; glycoprotein A33 (transmembrane); A33; cell surface A33 antigen; transmembrane glycoprotein A33; α-sar; α-sarcin
- Gene ID
- 10223
- UniProt ID
- Q99795
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| MOB-2119z | Mouse Anti-GPA33 Recombinant Antibody (MOB-2119z) | IHC, WB | Mouse IgG1 |
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| HPAB-1717-FY | Human Anti-GPA33 Recombinant Antibody (clone 263A17) | ELISA | Human IgG |
| FAMAB-1151CQ | Human Anti-GPA33 Recombinant Antibody (clone huA33) | FC, Cyt, IHC | Human IgG1 |
| FAMAB-0161WJ | Human Anti-GPA33 Recombinant Antibody (clone A2) | ELISA, WB, FC, IF | Human IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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