Recombinant Human Anti-HGFA Antibody (Fab40.deltaTrp) (CAT#: PABX-083)

Anti-HGFA antibody is a Human antibody of IgG class that binds to an HGFA. This antibody is a potent inhibitor of HGFA catalytic activity.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Figure 1 Superposition of the 99-loop of HGFA/Fab40.DTrp with the 99-loop of HGFA.

Figure 1 Superposition of the 99-loop of HGFA/Fab40.DTrp with the 99-loop of HGFA.

The conformation of the 99-loop (mustard) reverts almost back to the competent state in the Fab40.DTrp/HGFA complex structure. The CDR-H3 loop of Fab40. DTrp is highlighted in stick representation (orange).

Ganesan, R., Eigenbrot, C., Wu, Y., Liang, W. C., Shia, S., Lipari, M. T., & Kirchhofer, D. (2009). Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure, 17(12), 1614-1624.

Figure 2 Effects of Active Site Inhibitors on Antibody Binding to HGFA.

Figure 2 Effects of Active Site Inhibitors on Antibody Binding to HGFA.

(A, B, E, F) Surface plasmon resonance (BIAcore) measurements of binding to immobilized antibodies, Ab40 (A, B) or Ab40.DTrp (E, F), after coinjection of HGFA (A and E) or HGFA-KQLR (B and F) complex. (C) Competition binding (BIAcore) of HGFA to immobilized Ab40 in presence of different concentrations of KD1. (D) Competition binding ELISA measuring binding of HGFA to biotinylated KD1 in the presence of increasing antibody concentrations. Error bars represent SD.

Ganesan, R., Eigenbrot, C., Wu, Y., Liang, W. C., Shia, S., Lipari, M. T., & Kirchhofer, D. (2009). Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure, 17(12), 1614-1624.

Figure 3 Partial inhibition of chromogenic substrate, S-2266 hydrolysis (expressed as HGFA fractional activity ni /no) by Ab40 and lack of inhibition by Ab40.DTrp.

Figure 3 Partial inhibition of chromogenic substrate, S-2266 hydrolysis (expressed as HGFA fractional activity ni /no) by Ab40 and lack of inhibition by Ab40.DTrp.

Error bars represent standard deviation (SD).

Ganesan, R., Eigenbrot, C., Wu, Y., Liang, W. C., Shia, S., Lipari, M. T., & Kirchhofer, D. (2009). Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure, 17(12), 1614-1625.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human HGFA
  • Species Reactivity
  • Human
  • Clone
  • Fab40. deltaTrp
  • Applications
  • WB 1:1000-1:5000
    ELISA 1:3000-1:15000
    FuncS (assay dependent)

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • Assay-depended

Target

  • Alternative Names
  • HGFAC; HGF activator; HGFA; hepatocyte growth factor activator; anti-HGFA

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone Fab40. deltaTrp"

See other products for "HGFAC"

Human Antibody

Customer Reviews and Q&As

Submit a review or a question
There are currently no Customer reviews or questions for PABX-083. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.

For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Send Inquiry

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

© 2024 Creative Biolabs.
  • 0
  • 0
Cart

    Go to compare