Recombinant Human Anti-HGFA Antibody (Fab40.deltaTrp)
CAT#: PABX-083
Anti-HGFA antibody is a Human antibody of IgG class that binds to an HGFA. This antibody is a potent inhibitor of HGFA catalytic activity.
Published Data
Gene Expression
Figure 1 Superposition of the 99-loop of HGFA/Fab40.DTrp with the 99-loop of HGFA.
The conformation of the 99-loop (mustard) reverts almost back to the competent state in the Fab40.DTrp/HGFA complex structure. The CDR-H3 loop of Fab40. DTrp is highlighted in stick representation (orange).
Ganesan, R., Eigenbrot, C., Wu, Y., Liang, W. C., Shia, S., Lipari, M. T., & Kirchhofer, D. (2009). Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure, 17(12), 1614-1624.
Figure 2 Effects of Active Site Inhibitors on Antibody Binding to HGFA.
(A, B, E, F) Surface plasmon resonance (BIAcore) measurements of binding to immobilized antibodies, Ab40 (A, B) or Ab40.DTrp (E, F), after coinjection of HGFA (A and E) or HGFA-KQLR (B and F) complex. (C) Competition binding (BIAcore) of HGFA to immobilized Ab40 in presence of different concentrations of KD1. (D) Competition binding ELISA measuring binding of HGFA to biotinylated KD1 in the presence of increasing antibody concentrations. Error bars represent SD.
Ganesan, R., Eigenbrot, C., Wu, Y., Liang, W. C., Shia, S., Lipari, M. T., & Kirchhofer, D. (2009). Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure, 17(12), 1614-1624.
Figure 3 Partial inhibition of chromogenic substrate, S-2266 hydrolysis (expressed as HGFA fractional activity ni /no) by Ab40 and lack of inhibition by Ab40.DTrp.
Error bars represent standard deviation (SD).
Ganesan, R., Eigenbrot, C., Wu, Y., Liang, W. C., Shia, S., Lipari, M. T., & Kirchhofer, D. (2009). Unraveling the allosteric mechanism of serine protease inhibition by an antibody. Structure, 17(12), 1614-1625.
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RNA Expression
Figure 1 RNA cell line category: Cell line enriched (Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000109758-HGFAC
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG
- Specificity
- Tested positive against native Human HGFA
- Species Reactivity
- Human
- Clone
- Fab40. deltaTrp
- Applications
- WB 1:1000-1:5000
ELISA 1:3000-1:15000
FuncS (assay dependent)
Product Property
- Purity
- >95% by SDS-PAGE and HPLC analysis
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Applications
- Application Notes
- Assay-depended
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-463 | Recombinant Anti-human HGFAC Antibody | WB, ELISA, IHC, FuncS | IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-463-F(E) | Recombinant Anti-human HGFAC Antibody Fab Fragment | FC, WB, FuncS | Fab |
| MHH-463-F(E) | Recombinant Human Anti-human HGFAC Antibody Fab Fragment | WB, IP, FuncS | Fab |
| PFBL-133 | Recombinant Human Anti-HGFAC Antibody Fab Fragment (Ab40) | WB, ELISA, FuncS | Fab |
| PFBZ-058 | Human Anti-HGFAC Recombinant Antibody (clone Ab58); Fab Fragment | ELISA, Block | Human Fab |
| PFBW-058 | Human Anti-HGFAC Recombinant Antibody (clone Ab75); Fab Fragment | ELISA, Block | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-463-S(P) | Recombinant Anti-human HGFAC Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| MHH-463-S(P) | Recombinant Human Anti-human HGFAC Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| PSBL-133 | Recombinant Human Anti-HGFAC Antibody scFv Fragment (Ab40) | WB, ELISA, FuncS | scFv |
| PSBZ-058 | Human Anti-HGFAC Recombinant Antibody (clone Ab58); scFv Fragment | ELISA, Block | Human scFv |
| PSBW-058 | Human Anti-HGFAC Recombinant Antibody (clone Ab75); scFv Fragment | ELISA, Block | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-079CT | Human Anti-HGFAC Recombinant Antibody (TAB-079CT) | ELISA | Humanized antibody |
| TAB-079CT-S(P) | Human Anti-HGFAC Recombinant Antibody; scFv Fragment (TAB-079CT-S(P)) | ELISA | Humanized scFv |
| TAB-079CT-F(E) | Human Anti-HGFAC Recombinant Antibody; Fab Fragment (TAB-079CT-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABX-083-S (P) | Recombinant Human Anti-HGFA Antibody scFv Fragment (Fab40.deltaTrp) | WB, ELISA, Neut, FuncS | scFv |
| PABX-083-F (E) | Recombinant Human Anti-HGFA Antibody Fab Fragment (Fab40.deltaTrp) | WB, ELISA, Neut, FuncS | Fab |
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