Recombinant Human Antibody (N60-I3) is capable of binding to HIV-1 env A32-like epitope, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 env mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 env mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.
Figure 1 FRET histograms of donor (A488)-labeled Fabs, acceptor (A568)-labeled Fabs, and full-length single-chain gp120BaL-CD4 complex (FLSC) in solution as determined by the FRET-FCS approach.
Data in panels A and B indicate ∼20% FRET efficiency for A488-labeled C11 Fab and A568-labeled A32 Fab or N60-i3 Fab bound to FLSC, respectively. Panels D and E do not show detectable FRET signals for A488-labeled A32 Fab and A568-labeled N60-i3 Fab or JR4 Fab in the presence of FLSC in solution. From the FCS measurements, the diffusion coefficient of dye-labeled Fab is ∼83 μm2/s. This diffusion has been significantly decreased upon binding two Fabs to FLSC. The diffusion coefficient for this immune complex is ∼36 μm2/s.
Gohain, N., Tolbert, W. D., Acharya, P., Yu, L., Liu, T., Zhao, P.,... & Martin, L. (2015). Co-crystal structures of antibody N60-i3 and antibody JR4 in complex with gp120 define more Cluster A epitopes involved in effective antibody-dependent effector function against HIV-1. Journal of virology, JVI-01232.
Figure 2 MAb A32/C11 competition of N60-i3/JR4 Fab binding as measured by a surface plasmon resonance competition assay.
MAb A32 (A and B) and MAb C11 (B, C, and D) were immobilized on a protein A chip, and FLSC antigen was loaded to form a competitor antibody-antigen complex. Various concentrations of Fab MAb were tested. N60-i3 (A and C), JR4 (B and D), and A32 (E) were then passed over the chip. (F) Sensorgrams of the concentration series are shown. Binding affinities for MAbs A32, N60-i3, and JR4 to FLSC in the absence (blue bars) and presence (red bars) of C11 IgG were calculated with the BIAevaluation software.
Gohain, N., Tolbert, W. D., Acharya, P., Yu, L., Liu, T., Zhao, P.,... & Martin, L. (2015). Co-crystal structures of antibody N60-i3 and antibody JR4 in complex with gp120 define more Cluster A epitopes involved in effective antibody-dependent effector function against HIV-1. Journal of virology, JVI-01232.
Figure 3 Representative ADCC curves for MAbs N60-i3 and JR4. ADCC assays were performed using CEM-NKr-CCR5 target cells sensitized with gp120 of HIV-1BaL isolate (A) or spinoculated with AT-2-inactivated BaL viruses (B). MAb palivizumab (Synagis; MedImmune, Inc.) was included as a control.
Gohain, N., Tolbert, W. D., Acharya, P., Yu, L., Liu, T., Zhao, P.,... & Martin, L. (2015). Co-crystal structures of antibody N60-i3 and antibody JR4 in complex with gp120 define more Cluster A epitopes involved in effective antibody-dependent effector function against HIV-1. Journal of virology, JVI-01232.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-474 | Recombinant Anti-HIV-1 Env Antibody | ELISA, WB, IF, FuncS | IgG |
PABL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124) | WB, Neut, FuncS | Human IgG |
PABL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22) | WB, ELISA, Neut, FuncS | Human IgG |
PABL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L) | WB, ELISA, IF, FuncS | Human IgG |
PABL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11) | WB, ELISA, FuncS | Human IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
CAT | Product Name | Application | Type |
---|---|---|---|
PFBL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124); Fab Fragment | WB, Neut, FuncS | Human Fab |
PFBL-511 | Human Anti-HIV-1 Env Recombinant Antibody (clone 17B); Fab Fragment | Neut | Human Fab |
PFBL-512 | Recombinant Human Anti-HIV-1 Env Antibody Fab Fragment (A32) | WB, FuncS | Fab |
PFBL-513 | Monkey Anti-HIV-1 Env Recombinant Antibody (clone GE356); Fab Fragment | WB, FuncS | Monkey Fab |
PFBL-516 | Human Anti-HIV-1 Env Recombinant Antibody; Fab Fragment (PFBL-516) | Neut | Human Fab |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0122-WJ-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone B358.II); scFv Fragment | ELISA, WB | Human scFv |
PABJ-0103-S(P) | Rabbit Anti-HIV-1 Env Recombinant Antibody (clone E70); scFv Fragment | ELISA, Neut | Rabbit scFv |
PABJ-0140-S(P) | Human Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (PABJ-0140-S(P)) | ELISA, Neut | Human scFv |
PABJ-0141-S(P) | Human Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (PABJ-0141-S(P)) | ELISA, Neut | Human scFv |
PABJ-0147-S(P) | Human Anti-HIV-1 Env Recombinant Antibody (clone ACS202); scFv Fragment | ELISA, Neut | Human scFv |
There are currently no Customer reviews or questions for PABL-150. Click the button above to contact us or submit your feedback about this product.
View the frequently asked questions answered by Creative Biolabs Support.
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.