Human Anti-HIV-1 gp160 Recombinant Antibody (clone PGT128) (CAT#: PABL-179)

Figure 1 High-mannose patch antibodies were tested for competition with sCD4 on isolate JR-FL E168K/N192A Env displayed on the surface of 293T cells. The CD4 binding site antibody 12A12 was included as a positive control and the V3-specific antibody F425 was included as a negative control.

Sok, D., Pauthner, M., Briney, B., Lee, J. H., Saye-Francisco, K. L., Hsueh, J.,... & Bastidas, R. (2016). A prominent site of antibody vulnerability on HIV envelope incorporates a motif associated with CCR5 binding and its camouflaging glycans. Immunity, 45(1), 31-45.

Figure 2 the functional stability of all proteins was tested by monitoring binding to bNAbs, VRC01 (CD4bs specific), and PGT128 (V3 loop glycan specific) before and after heating.

Proteins were incubated at 60 or 100 °C for 1 h and cooled down to room temperature to monitor binding to bNAbs VRC01 (left panel) and PGT128 (right panel) immobilized on the surface of a CM5 chip. Biacore response units were plotted after incubation at different temperatures to compare stabilities. Cyclically permuted gp120 trimers are stable at 60 °C showing similar binding before and after heating to VRC01 and PGT128 antibodies. They retained detectable binding to these antibodies even after incubation at 100 °C for 1 h.

Figure 1 Recombinant Anti-HIV env Antibody (PABL-179) in SDS-PAGE

SDS-PAGE analysis of PABL-179 in non-reduced (Lane 1) and β-mercaptoethanol reduced (Lane 2) conditions.

Figure 2 Recombinant Anti-HIV env Antibody (PABL-179) in HPLC

The purity of PABL-179 was greater than 95% as determined by SEC-HPLC.