Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (CAT#: MRO-961CQ)
This antibody is a mouse monoclonal antibody that is capable of binding to HIV-1 p24.
Specific Inquiry
ELISA
Figure 1 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in ELISA.
ELISA analysis of MRO-961CQ was performed by coating with recombinant HIV-1 p24 antigen, His tag.
WB
Figure 2 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in WB.
Western blot analysis of MRO-961CQ was performed by loading 0.1 µg (lane 1), 0.3 µg(lane 2), and 0.6 µg (lane 3)recombinant HIV-1 p24 antigen, His tag onto a 12% Tris-HCl polyacrylamide gel.
DB
Figure 3 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in Dot Blot.
Dot blot analysis of MRO-961CQ by coating with recombinant HIV-1 p24 antigen, His tag.
SDS-PAGE
Figure 4 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in SDS-PAGE.
SDS-PAGE analysis of MRO-961CQ in non-reduced (Lane 1, 1.5 μg) and β-mercaptoethanol-reduced (Lane 2, 1.5 μg) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 5 Mouse Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) (MRO-961CQ) in SEC-HPLC.
The purity of MRO-961CQ was 98.17% as determined by SEC-HPLC.
Specifications
- Immunogen
- HIV-1 p24 protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- HIV-1 p24
- Species Reactivity
- HIV-1
- Clone
- AG3.0
- Applications
- ELISA
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Human immunodeficiency virus type 1; HIV-1; Capsid protein p24; p24
- Gene ID
- 155030
- UniProt ID
- P04591
Related Resources
Tada, Takuya, et al. "Prophylaxis and treatment of SARS-CoV-2 infection by an ACE2 receptor decoy in a preclinical animal model." Iscience 26.2 (2023). https://doi.org/10.1016/j.isci.2023.106092
The study conducted by Takuya Tada and colleagues focuses on the development and testing of a high-affinity ACE2 receptor decoy to combat SARS-CoV-2 infection, including variants like Omicron. The decoy, a modified ACE2 ectodomain fused to an immunoglobulin heavy chain Fc region, was shown to significantly suppress virus loads in preclinical animal models. The decoy demonstrated potent neutralizing activity against various SARS-CoV-2 variants, both in vitro and in vivo, by preventing the virus from binding to the ACE2 receptor on host cells. This approach offers a promising prophylactic and therapeutic strategy against current and potentially future SARS-CoV-2 variants, given its ability to target the conserved ACE2 binding mechanism of the virus.
Creative Biolabs played a crucial role in this study by providing the anti-p24 monoclonal antibody AG3.0 (Cat#: MRO-961CQ). This antibody was essential for the virion-decoy pull-down assay, which involved binding decoy proteins to nickel-nitrilotriacetic acid-agarose beads, incubating with spike protein-pseudotyped virus, and analyzing bound virions on an immunoblot. The anti-p24 antibody AG3.0 enabled the detection of bound virions, facilitating the evaluation of the decoy protein's efficacy in neutralizing the virus. This contribution was significant as it provided a key reagent necessary for the successful execution and analysis of the assay.
More Infomation
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Human Antibody Products
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ScFv Antibody Products
Fab Antibody Products
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-186LC | Anti-HIV-1 p24 Recombinant Antibody (6A9/5) | ELISA | Human antibody |
| MRO-186LC-S(P) | Anti-HIV-1 p24 Recombinant Antibody scFv Fragment (6A9/5) | ELISA | Human antibody |
| MRO-186LC-F(E) | Anti-HIV-1 p24 Recombinant Antibody Fab Fragment (6A9/5) | ELISA | Human antibody |
| MRO-187LC-F(E) | Anti-HIV-1 p24 Recombinant Antibody Fab Fragment (6D9/4) | ELISA | Human antibody |
| MRO-188LC-F(E) | Anti-HIV-1 p24 Recombinant Antibody Fab Fragment (2E7/3) | ELISA | Human antibody |
Customer Reviews and Q&As
Customer Review
Q&As
Megan Roberts
Great for HIV Research
Great for HIV Research
The Anti-HIV-1 p24 Recombinant Antibody (clone AG3.0) has been excellent for our HIV research. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
15-Sep-23
James Brown
Effective in Immunoassays
Effective in Immunoassays
We incorporated this antibody into our immunoassays, and it has been a great asset. The specificity for HIV-1 p24 is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
19-Nov-22
Emily Harris
Consistent High-Quality Results
Consistent High-Quality Results
This antibody has proven to be consistent and reliable in our assays. The high-quality, reproducible results have significantly improved our experiments, making it a crucial part of our research toolkit. The strong affinity and specificity for HIV-1 p24 have been particularly beneficial.
20-Jun-21
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# MRO-961CQ, RRID: AB_3111553)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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