Mouse Anti-HIV Nef Recombinant Antibody (MRO-754CQ)
CAT#: MRO-754CQ
This antibody is a mouse monoclonal antibody that is capable of binding to HIV Nef.
Specifications
- Immunogen
- HIV Nef
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- HIV Nef
- Species Reactivity
- HIV
- Applications
- ELISA, IP, IF, WB
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- HIV Nef; Human Immunodeficiency Viruse Nef; Human Immunodeficiency Viruse; HIV Nef protein; HIV Nef-protein; p27; HIV1gp9; F-protein; Negative factor
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Effective For Nef Detection
The HIV Nef protein is central to our study of viral virulence factors. This mouse monoclonal antibody (MRO-754CQ) detects Nef reliably in Western Blot and ELISA applications. It helps us track the expression levels of this virulence factor in our infected cell culture models with high specificity.
Good For Immunofluorescence
We also utilized this antibody for Immunofluorescence to observe the intracellular localization of Nef and its effect on membrane signaling. The staining was distinct, and the antibody worked well in Immunoprecipitation assays to isolate Nef-associated complexes. It is a versatile tool for HIV pathogenesis research.
Q&As
-
What applications can this HIV Nef antibody be used for?
A: This mouse monoclonal antibody (IgG1, kappa) is capable of binding to HIV Nef and is validated for multiple applications, including Enzyme-linked Immunosorbent Assay (ELISA), Immunoprecipitation (IP), Immunofluorescence (IF), and Western Blot (WB).
-
What is the target protein for this antibody?
A: The antibody targets the HIV Nef protein, a multifunctional virulence factor that perturbs intracellular membranes and signaling. Nef is also known as p27, F-protein, or Negative factor. The antibody recognizes the Nef protein from the Human Immunodeficiency Virus.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "HIV Nef"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-472 | Recombinant Anti-HIV Nef Antibody | ELISA, WB, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-472-F(E) | Recombinant Anti-HIV Nef Antibody Fab Fragment | WB, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-472-S(P) | Recombinant Anti-HIV Nef Antibody scFv Fragment | IP, IF, Biosensors, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-472 | Recombinant Human Anti-HIV Nef Antibody | FC, Neut, Biosensors, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-472-F(E) | Recombinant Human Anti-HIV Nef Antibody Fab Fragment | WB, Dot, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-472-S(P) | Recombinant Human Anti-HIV Nef Antibody scFv Fragment | ELISA, WB, IF, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PNBL-022 | Recombinant Anti-HIV-1 nef VHH Single Domain Antibody (PNBL-022) | Inhib, WB, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0821 | PE-A*11:01/HIV NEF (AVDLSHFLK) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0822 | APC-A*11:01/HIV NEF (AVDLSHFLK) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0823 | FITC-A*11:01/HIV NEF (AVDLSHFLK) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0824 | A*11:01/HIV NEF (AVDLSHFLK) MHC Tetramer | FCM, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF256 | Mafa-A1*063/SIV nef HW8 (HPAQTSQW) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-FY777 | Mouse Anti-nef Recombinant Antibody (clone 2506W0672) | ELISA | Mouse IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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