Recombinant Mouse anti-Human MARCKS Monoclonal antibody (EML519)
CAT#: MOB-1205CT
Recombinant Mouse monoclonal antibody to Human MARCKS, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54820/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/1237_G1_4_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (AF22, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000277443-MARCKS
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Specifications
- Immunogen
- Recombinant fragment: GAQFSKTAAK GEAAAERPGE AAVASSPSKA NGQENGHVKV NGDASPAAAE SGAKEELQAN GSAP, corresponding to amino acids 2-66 of Human MARCKS
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- EML519
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- Batch dependent within range:0.40 -0.5 mg/ml
- Buffer
- Preservative: None PBS, pH 7.2
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- MARCKS; myristoylated alanine-rich protein kinase C substrate; MACS; 80K-L; PKCSL; PRKCSL; myristoylated alanine-rich C-kinase substrate; phosphomyristin; protein kinase C substrate, 80 kDa protein, light chain; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L);
- Gene ID
- 4082
- UniProt ID
- P29966
- Protein Refseq
- NP_002347
- Function
- actin filament binding; calmodulin binding; protein kinase C binding;
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MARCKS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3120z | Mouse Anti-MARCKS Recombinant Antibody (clone 41C9) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-060CQ | Rabbit Anti-MARCKS (p Ser156) Antibody | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2165 | Hi-Affi™ Recombinant Rabbit Anti-MARCKS Monoclonal Antibody (DS2165AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY315 | Mouse Anti-NHP MARCKS Recombinant Antibody (clone 5F9) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4259 | Anti-MARCKS Immunohistochemistry Kit | IHC |
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