Mouse Anti-NHP MARCKS Recombinant Antibody (clone 5F9)
CAT#: VS-1024-XY315
Specially formulated for the detection of MARCKS, this mouse monoclonal antibody exhibits reactivity with human and non-human primate samples. The immunogen is a full-length human MARCKS protein, providing excellent specificity. With validated applications in Western blot (WB) and immunofluorescence (IF), this antibody is an invaluable tool for exploring MARCKS expression and functionality, making it essential for a wide range of research and diagnostic investigations.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54820/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/1237_G1_4_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (AF22, TIME)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000277443-MARCKS
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Specifications
- Immunogen
- Full length human MARCKS
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MARCKS
- Species Reactivity
- Human, Non-human primate
- Clone
- 5F9
- Applications
- WB, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:20, IF: 1:20
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
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- Product Name
Isotype Control
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Secondary Antibody
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- Product Name
See other products for "MARCKS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3120z | Mouse Anti-MARCKS Recombinant Antibody (clone 41C9) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-1205CT | Recombinant Mouse anti-Human MARCKS Monoclonal antibody (EML519) | ICC, IF, WB | |
| MOB-060CQ | Rabbit Anti-MARCKS (p Ser156) Antibody | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2165 | Hi-Affi™ Recombinant Rabbit Anti-MARCKS Monoclonal Antibody (DS2165AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4259 | Anti-MARCKS Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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