Mouse Anti-NHP MARCKS Recombinant Antibody (clone 5F9) (CAT#: VS-1024-XY315)
Specially formulated for the detection of MARCKS, this mouse monoclonal antibody exhibits reactivity with human and non-human primate samples. The immunogen is a full-length human MARCKS protein, providing excellent specificity. With validated applications in Western blot (WB) and immunofluorescence (IF), this antibody is an invaluable tool for exploring MARCKS expression and functionality, making it essential for a wide range of research and diagnostic investigations.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54820/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54820/if_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/1237_G1_4_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/1237_G1_4_selected.jpg
Figure 3 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_9_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_9_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_8_5.jpg
Figure 5 Testis
(Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_6_6.jpg
(Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67595/158237_A_6_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (AF22, TIME)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000277443-MARCKS
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000277443-MARCKS
Specifications
- Immunogen
- Full length human MARCKS
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MARCKS
- Species Reactivity
- Human, Non-human primate
- Clone
- 5F9
- Applications
- WB, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:20, IF: 1:20
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3120z | Mouse Anti-MARCKS Recombinant Antibody (clone 41C9) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-1205CT | Recombinant Mouse anti-Human MARCKS Monoclonal antibody (EML519) | ICC/IF, WB | |
| MOB-060CQ | Rabbit Anti-MARCKS (p Ser156) Antibody | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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