Recombinant Mouse anti-Human POU2F1 Monoclonal antibody (43H4) (CAT#: MOB-2258CT)
Recombinant Mouse Monoclonall antibody to Human POU2F1, expressed in Chinese Hamster Ovary cells(CHO).
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58559/1259_D5_1_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58559/1259_D5_1_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows moderate nuclear positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows moderate nuclear positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_B_9_5.jpg
(Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_B_9_5.jpg
Figure 4 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_7_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_7_3.jpg
Figure 5 Liver
(Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_8_4.jpg
(Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_8_4.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_9_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_9_5.jpg
Figure 7 Urinary bladder
(Urothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_6_5.jpg
(Urothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_6_5.jpg
Figure 8 Testis
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_6_6.jpg
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_6_6.jpg
Figure 9 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_8_8.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143190-POU2F1/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143190-POU2F1/subcellular
Specifications
- Immunogen
- Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human Oct1.
- Host Species
- Mouse
- Specificity
- Ubiquitous. Isoform 2 is lymphocyte-specific.
- Species Reactivity
- Human
- Clone
- 43H4
- Applications
- Used for immunoassay techniques such as: Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Immunogen affinity purified
- Format
- Liquid
- Buffer
- Preservative: 0.02% Sodium Azide. Constituents: 1% BSA, PBS, pH 7.4
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- POU2F1; POU class 2 homeobox 1; OCT1; OTF1; oct-1B; POU domain, class 2, transcription factor 1; NF-A1; OTF-1; oct-1; octamer-binding protein 1; Octamer-binding transcription factor-1; octamer-binding transcription factor 1;
- Gene ID
- 5451
- UniProt ID
- P14859
- Protein Refseq
- NP_001185712
- Function
- protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity;
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1125-CN | Recombinant Rabbit Anti-POU2F1 Monoclonal Antibody (JG62-35) | WB, IHC | Rabbit IgG |
| MOB-0147F | Mouse Anti-POU2F1 Recombinant Antibody (clone 7G1) | WB, IHC-P, IF | Mouse IgG |
| VS3-CJ657 | Mouse Anti-POU2F1 Recombinant Antibody (VS3-CJ657) | WB, IHC | Mouse IgG |
| VS4-CJ80 | Recombinant Mouse Anti-POU2F1 Antibody (VS4-CJ80) | ELISA, WB | Mouse IgG |
| VS3-FY1169 | Recombinant Rabbit Anti-POU2F1 Antibody (clone R03-1K1) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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