Mouse Anti-POU2F1 Recombinant Antibody (VS3-CJ657)
CAT#: VS3-CJ657
This product is a mouse antibody that recognizes human, mouse, and rat POU2F1.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line CACO-2
Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58559/1259_D5_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows moderate nuclear positivity in germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_9_5.jpg
Normal Tissue
Figure 7 Urinary bladder
Urothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_6_5.jpg
Normal Tissue
Figure 8 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143190-POU2F1/subcellular
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Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, Mouse, Rat POU2F1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Fractional precipitation
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, PBS, pH 7.4.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunohistochemistry.
Target
- Alternative Names
- OCT1; OTF1; Oct1Z; oct-1B
- Gene ID
- 5751
- UniProt ID
- P14859
- Sequence Similarities
- Belongs to the POU transcription factor family. Class-2 subfamily.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by PRKDC.
- Protein Refseq
- NP_001185712.1; NP_001185715.1; NP_002688.3
- Function
- Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.
(Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| MOB-2258CT | Recombinant Mouse anti-Human POU2F1 Monoclonal antibody (43H4) | WB | |
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| VS3-WK508 | Rabbit Anti-POU2F1 Recombinant Antibody (clone JG62-35) | WB, ELISA | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5581 | Anti-POU2F1 Immunohistochemistry Kit | IHC |
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