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    Mouse Anti-POU2F1 Recombinant Antibody (VS3-CJ657) (CAT#: VS3-CJ657)

    This product is a mouse antibody that recognizes human, mouse, and rat POU2F1.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line CACO-2
    Figure 1 IF staining of human cell line CACO-2
    (Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/58559/1259_D5_1_selected.jpg
    Figure 2 IHC staining of human lymph node
    Figure 2 IHC staining of human lymph node
    (Immunohistochemical staining of human lymph node shows moderate nuclear positivity in germinal center cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Endothelial cells
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_B_9_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Hepatocytes
    Staining: Low
    Intensity: Weak
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_8_4.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear
    Cells in tubules
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_9_5.jpg
    Figure 7 Urinary bladder
    Figure 7 Urinary bladder
    (Urothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_6_5.jpg
    Figure 8 Testis
    Figure 8 Testis
    (Sertoli cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Spermatogonia cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_6_6.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/64323/143883_A_8_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Low cell line specificity
    Figure 10 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000143190-POU2F1/subcellular

    Specifications

    • Immunogen
    • Synthetic peptide
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human, Mouse, Rat POU2F1
    • Species Reactivity
    • Human, Mouse, Rat
    • Applications
    • WB, IHC
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Fractional precipitation
    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • 50% Glycerol, PBS, pH 7.4.
    • Preservative
    • 0.02% Sodium Azide
    • Storage
    • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody has been tested for use in Western Blot, Immunohistochemistry.

    Target

    • Alternative Names
    • OCT1; OTF1; Oct1Z; oct-1B
    • Sequence Similarities
    • Belongs to the POU transcription factor family. Class-2 subfamily.
    • Cellular Localization
    • Nucleus
    • Post Translation Modifications
    • Phosphorylated by PRKDC.
    • Function
    • Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.
      (Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "POU2F1"

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    IgG Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
    CAT Product Name Application Type
    MOB-2258CT Recombinant Mouse anti-Human POU2F1 Monoclonal antibody (43H4) WB
    MRO-1125-CN Recombinant Rabbit Anti-POU2F1 Monoclonal Antibody (JG62-35) WB, IHC Rabbit IgG
    VS3-WK508 Rabbit Anti-POU2F1 Recombinant Antibody (clone JG62-35) WB, ELISA Rabbit IgG
    VS4-CJ80 Recombinant Mouse Anti-POU2F1 Antibody (VS4-CJ80) ELISA, WB Mouse IgG
    VS3-FY1169 Recombinant Rabbit Anti-POU2F1 Antibody (clone R03-1K1) WB Rabbit IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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