Recombinant Mouse anti-Human PPARG Monoclonal antibody (TQN302)
CAT#: MOB-2265CT
Recombinant Mouse Monoclonall antibody to Human PPARG, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51239/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_5.jpg
Normal Tissue
Figure 6 Placenta
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_2_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BEWO, CAPAN-2, HSkMC, PC-3, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132170-PPARG/subcellular
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Specifications
- Immunogen
- Baculovirus-expressed recombinant fragment, corresponding to amino acids 3-105 of Human PPAR gamma 1+2.
- Host Species
- Mouse
- Specificity
- Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
- Species Reactivity
- Human
- Clone
- TQN302
- Applications
- Used for immunoassay techniques such as: Immunohistochemistry paraffin embedded sections; Western Blot; Enzyme-linked Immunosorbent Assay; Immunoprecipitation; ChImmunoprecipitation; GSA
- Conjugate
- Unconjugated
Product Property
- Format
- Liquid
- Buffer
- Physiological saline. Preservative: 0.1% Sodium Azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- PPARG; peroxisome proliferator-activated receptor gamma; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated nuclear receptor gamma variant 1;
- Gene ID
- 5468
- UniProt ID
- P37231
- Protein Refseq
- NP_005028
- Function
- DNA binding; activating transcription factor binding; arachidonic acid binding; drug binding; enzyme binding; ligand-dependent nuclear receptor activity; ligand-dependent nuclear receptor activity; metal ion binding; prostaglandin receptor activity; prote
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| BRD-0429MZ | Chicken Anti-PPARγ Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
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| MRO-2129-CN | Rabbit Anti-PPARG Polyclonal Antibody (MRO-2129-CN) | WB, IF, IHC, FC | Rabbit IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1705 | Anti-PPARG Immunohistochemistry Kit | IHC | |
| VS-0525-XY5591 | Anti-Human PPARG Immunohistochemistry Kit | IHC |
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