Rabbit Anti-PPARG Polyclonal Antibody (MRO-2129-CN)
CAT#: MRO-2129-CN
This antibody is a rabbit polyclonal antibody that is capable of binding to PPARG.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51239/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_5.jpg
Normal Tissue
Figure 6 Placenta
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_2_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BEWO, CAPAN-2, HSkMC, PC-3, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132170-PPARG/subcellular
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Specifications
- Immunogen
- Recombinant protein within human PPAR gamma aa 250-450.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PPARG (Peroxisome proliferator activated receptor gamma)
- Species Reactivity
- Human, Mouse, Rat
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunohistochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein affinity purified
- Format
- Liquid
- Buffer
- 1*TBS, pH 7.4, 0.5% BSA, 50% Glycerol
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Target
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Related Signaling Pathways
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Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PPARG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1668z | Mouse Anti-PPARG Recombinant Antibody (clone 28C5) | WB, ELISA, IF, IHC | Mouse IgG1 |
| MRO-1258-CN | Recombinant Rabbit Anti-PPARG Monoclonal Antibody (JF101-4) | WB | Rabbit IgG |
| VS4-CJ84 | Recombinant Mouse Anti-PPARG Antibody (VS4-CJ84) | ELISA, WB, ICC | Mouse IgG1 |
| VS13-YC920 | Rabbit Anti-PPARG Recombinant Antibody (VS13-YC920) | WB | Rabbit IgG |
| VS7-0425-WR656 | Mouse Anti-PPARG Monoclonal Antibody (clone 3A4A9) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0429MZ | Chicken Anti-PPARγ Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1705 | Anti-PPARG Immunohistochemistry Kit | IHC | |
| VS-0525-XY5591 | Anti-Human PPARG Immunohistochemistry Kit | IHC |
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