Mouse Anti-PPARG Recombinant Antibody (clone 28C5)
CAT#: MOB-1668z
This product is a mouse antibody that recognizes human PPARG. It can be used in the following applications: WB, ELISA, IF, IHC.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51239/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Neuronal cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_5.jpg
Normal Tissue
Figure 6 Placenta
Trophoblastic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_2_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4282/15105_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, BEWO, CAPAN-2, HSkMC, PC-3, RT4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132170-PPARG/subcellular
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Specifications
- Immunogen
- Human Peroxisome Proliferator-Activated Receptor Gamma
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PPARG
- Species Reactivity
- Human
- Clone
- 28C5
- Applications
- WB, ELISA, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- PPARG; Peroxisome Proliferator-Activated Nuclear Receptor Gamma Variant 1; GLM1; PPAR-Gamma; PPARG2; CIMT1; Peroxisome Proliferative Activated Receptor, Gamma; Peroxisome Proliferator-Activated Receptor Gamma; PPARG1; NR1C3
- UniProt ID
- P37231
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Related Signaling Pathways
mTOR Signaling Pathway
Related Diseases
Throid Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PPARG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0429MZ | Chicken Anti-PPARγ Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1258-CN | Recombinant Rabbit Anti-PPARG Monoclonal Antibody (JF101-4) | WB | Rabbit IgG |
| MRO-2129-CN | Rabbit Anti-PPARG Polyclonal Antibody (MRO-2129-CN) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-QX899 | Mouse Anti-PPARG Recombinant Antibody (clone 3G3-E2-H10) | WB | Mouse IgG2a |
| VS3-WK423 | Rabbit Anti-PPARG Recombinant Antibody (clone JF101-4) | WB | Rabbit IgG |
| VS4-CJ84 | Recombinant Mouse Anti-PPARG Antibody (VS4-CJ84) | ELISA, WB, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1705 | Anti-PPARG Immunohistochemistry Kit | IHC | |
| VS-0525-XY5591 | Anti-Human PPARG Immunohistochemistry Kit | IHC |
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