Recombinant Mouse anti-Human SOX17 Monoclonal antibody (4C20)
CAT#: MOB-1905CT
Recombinant Mouse monoclonal antibody to Human SOX17, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25594/if_selected.jpg
Normal Tissue
Figure 2 Endometrium 1
High expression in endometrium 1
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68399/154520_B_5_3_val_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68399/154520_A_9_3.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Round or early spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/68399/154520_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enriched (EFO-21)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164736-SOX17
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Specifications
- Immunogen
- Recombinant human SOX17 protein (amino acids 177-414), expressed in E. coli.
- Host Species
- Mouse
- Antibody Isotype
- IgG1
- Species Reactivity
- Human
- Clone
- 4C20
- Applications
- Used for immunoassay techniques such as: Flow Cytometry; Immunocytochemistry/Immunofluorescence; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 0.8 mg/ml
- Buffer
- Preservative: 0.02% Sodium Azide Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.3
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Target
- Alternative Names
- SOX17; SRY (sex determining region Y)-box 17; VUR3; transcription factor SOX-17; SRY-related HMG-box transcription factor SOX17;
- Gene ID
- 64321
- UniProt ID
- Q9H6I2
- Protein Refseq
- NP_071899
- Function
- beta-catenin binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity; transcription coactivator activity; transcription
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Excellent Nuclear Staining
We used the Anti-Human SOX17 Monoclonal Antibody (Clone 4C20) for immunocytochemistry on differentiated stem cells. The antibody gave a strong and specific nuclear signal, confirming the endodermal lineage commitment of our cells. The staining was clean with very low background, which made image analysis straightforward. A highly recommended antibody for developmental biology research.
Precise Flow Cytometry
This antibody worked very well in flow cytometry to quantify SOX17-positive cell populations. The separation between positive and negative populations was distinct, allowing for accurate gating. The recombinant production ensures that we can rely on the same clone specificity for all our future experiments investigating cell fate determination.
Q&As
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What clone is this SOX17 antibody derived from?
A: This Recombinant Mouse monoclonal antibody corresponds to clone 4C20. It is generated against a recombinant fragment of Human SOX17 amino acids 177-414.
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Can I use this antibody for immunofluorescence staining?
A: Yes, this antibody is suitable for Immunocytochemistry/Immunofluorescence (ICC/IF). It is also validated for Flow Cytometry (FC) and Western Blot (WB), making it a versatile tool for studying SOX17 expression and localization.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SOX17"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3988z | Mouse Anti-SOX17 Recombinant Antibody (clone 24A10) | WB, ICC, IF, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1096 | Rabbit Anti-SOX17 Recombinant Antibody (VS13-YC1096) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6818 | Anti-SOX17 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6819 | Anti-Mouse SOX17 Immunohistochemistry Kit | IHC |
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