Recombinant Mouse anti-Human SPTB Monoclonal antibody (EC3)
CAT#: MOB-0254CT
Recombinant Mouse monoclonal antibody to Human SPTB, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line K-562
Immunofluorescent staining of human cell line K-562 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3394/1854_H8_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows membranous positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16232/ihc_selected.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16232/133364_A_9_5.jpg
Normal Tissue
Figure 4 High expression in skeletal muscle
Myocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16232/133364_B_1_6.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/16232/133364_B_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HEL, K-562, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000070182-SPTB
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Specifications
- Immunogen
- The immunogen is a preparation of ghost proteins from human red blood cells, representing the membrane proteins.
- Host Species
- Mouse
- Specificity
- DMABT-26358MH is specific to the erythroid beta 1 Spectrin.
- Species Reactivity
- Human
- Clone
- EC3
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunoprecipitation; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 0.1 mg/ml
- Buffer
- Preservative: 0.1% Sodium Azide Constituents: 1% BSA, PBS
- Storage
- Store at +4°C.
Target
- Alternative Names
- SPTB; spectrin, beta, erythrocytic; EL3; HS2; SPH2; HSPTB1; spectrin beta chain, erythrocytic; beta-I spectrin; membrane cytoskeletal protein; spectrin beta chain, erythrocyte;
- Gene ID
- 6710
- UniProt ID
- P11277
- Protein Refseq
- NP_000338
- Function
- actin binding; actin filament binding; protein binding; structural constituent of cytoskeleton;
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Specific To Erythroid Spectrin
We needed an antibody specific for erythroid beta 1 Spectrin, and clone EC3 was the perfect match. It performed exceptionally well in immunofluorescence assays, clearly labeling the membrane cytoskeletal structures we were investigating.
Versatile Application Range
We have successfully used this antibody for both immunoprecipitation and Western Blotting. It binds specifically to the ghost proteins of human red blood cells, making it a versatile tool for our hematological research projects.
Q&As
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What is the specificity of the EC3 clone?
A: The EC3 clone (also known as DMABT-26358MH) is specific to the erythroid beta 1 Spectrin. It was raised against ghost proteins of human red blood cells and does not cross-react with non-erythroid spectrins.
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What applications can I use this antibody for?
A: This recombinant mouse monoclonal antibody is versatile and can be used for Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), and Western Blot (WB). It is a valuable tool for studying cytoskeletal organization in erythrocytes.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SPTB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2424z | Mouse Anti-SPTB Recombinant Antibody (clone 40G8) | WB, ICC, IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3401 | Hi-Affi™ Recombinant Rabbit Anti-SPTB Monoclonal Antibody (DS3401AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-1261CQ | Human Anti-SPTB Recombinant Antibody (clone RG/26) | WB, IF, IHC | Human IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2166 | Anti-SPTB Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6882 | Anti-Mouse SPTB Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY6881 | Anti-Human SPTB Immunohistochemistry Kit | IHC |
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