Mouse Anti-IL31 Recombinant Antibody (clone 34D03) (CAT#: HPAB-0132-LSX)

Recombinant monoclonal antibody that specifically binds to at least one of canine Interleukin-31 (IL-31) or feline IL-31. Clone 34D03 can be in the form of diagnostic and/or veterinary compositions useful for treating a pruritic and/or allergic condition in dogs or cats.

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WB

Figure 1 Western blots with full length and truncated canine IL-31 proteins.

Individual nitrocellulose blots were probed with A) anti-His B) 34D03 and C) 11E12 antibodies. Lanes 1-9 of the blots correspond to the following: Lane 1-pre-stained protein standards (Seeblue plus 2, Invitrogen Corp., Carlsbad, Calif.); Lane 2-full-length canine IL-31; Lane 3-N-terminal truncation −20N; Lane 4-N-terminal truncation −40N; Lane 5-N-terminal truncation −60N; Lane 6-C-terminal truncation −20C; Lane 7-C-terminal truncation −40C; Lane 8-C-terminal truncation −60C; and Lane 9-beta-galactosidase (lacZ). Note: full length IL-31 and proteins with C-terminal truncations (−20, −40C, and −60C) showed no detectable expression under these conditions.

WB

Figure 2 Western blots with truncated canine IL-31 proteins.

Individual nitrocellulose blots were probed with A) anti-His B) 11E12 and C) 34D03 antibodies. Lanes 1-5 of the blots correspond to the following: Lane 1-pre-stained protein standards; Lane 2-C-terminal truncations at positions 20-122; Lane 3-C-terminal truncations at positions 20-100; Lane 4-C-terminal truncations at positions 20-80; and Lane 5-beta-galactosidase (lacZ).

WB

Figure 3 Western blots with lysates of E. coli strains expressing canine IL-31 with alanine substituted for each amino acid position (76-122).

Individual nitrocellulose blots were probed with anti-His, 11E12 and, 34D03 antibodies, as shown in the Figure.

WB

Figure 4 Western blot of feline and canine IL-31 under reducing conditions probed with felinized antibody 34D03.

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Figure 5 A section of Western blots with double and triple mutations in canine IL-31. −20N protein lysate was run as a positive control.

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Figure 6 Western blots with IL-31 proteins. Individual nitrocellulose blots were probed with A) anti-His B) 11E12 and C) 34D03 antibodies.

Note—Canine IL-31 (CHO) does not contain a 6-His tag.

Inhib

Figure 7 A graph showing the inhibition of canine IL-31 induced pSTAT signaling in canine DH82 monocytes comparing felinized and caninized antibody 34D03.


Specifications

  • Immunogen
  • Recombinant canine IL-31
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Canine IL31
  • Species Reactivity
  • Canine
  • Clone
  • 34D03
  • Applications
  • ELISA, WB, Inhib
  • Related Disease
  • Pruritic condition or an allergic condition in dogs or cats

Applications

  • Application Notes
  • This antibody has been tested for use in Western Blot and Inhibition.

Target

  • Alternative Names
  • IL31; interleukin 31

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "IL31"

See other products for "Clone 34D03"


For Research Use Only. Not For Clinical Use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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