Mouse Anti-LAM Recombinant Antibody (clone CS-35); Fab Fragment (CAT#: PFBW-158)
LAM is an important immunodiagnostic target for detecting active infection with Tuberculosis (TB). This recombinant mouse antibody Fab fragment (CS-35) is capable of binding to LAM. CS-35 may interact with additional Araf residues connected through α-(1→5)-glycosidic linkages to the reducing end of residue A. Such residues are found in natural LAM and may enhance binding affinity to CS-35.
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SDS-PAGE
Figure 1 Anti-LAM Recombinant Antibody (clone CS-35); Fab Fragment (PFBW-158) in SDS-PAGE
SDS-PAGE analysis of PFBW-158 in β-mercaptoethanol-reduced (Lane 2) and non-reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 27 kDa and 25 kDa, respectively.
WB
Figure 2 Anti-LAM Recombinant Antibody (clone CS-35); Fab Fragment (PFBW-158) in WB
Western blot analysis of PFBW-158 was performed by loading Native M. Tuberculosis LAM-Antigen.
PFBW-158 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-conjugated Goat Anti-Mouse IgG ( H+L).
Lane 1: Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (1 μg)
Lane 3: Reducing Antigen (2 μg)
ELISA
Figure 3 Anti-LAM Recombinant Antibody (clone CS-35); Fab Fragment (PFBW-158) in ELISA
ELISA analysis of PFBW-158 was performed by coating with Native M. Tuberculosis LAM-Antigen. Then blocked with BSA and incubated with PFBW-158. The HRP-conjugated Mouse Anti-His as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Specifications
- Immunogen
- Lipoarabinomannan
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse Fab
- Clone
- CS-35
- Applications
- ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- LAM; Lipoarabinomannan
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
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IgM Antibody Products
Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABW-158 | Mouse Anti-LAM Recombinant Antibody (clone CS-35) | ELISA, IF, FC | Mouse IgG |
| HPAB-M0560-YC | Human Anti-LAM Recombinant Antibody (HPAB-M0560-YC) | ELISA, WB, Microarray, Block | Human IgG, κ |
| HPAB-M0561-YC | Human Anti-LAM Recombinant Antibody (HPAB-M0561-YC) | ELISA, WB, Microarray, Block | Human IgM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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