Human Anti-LAM Recombinant Antibody; Fab Fragment (HPAB-M0560-YC-F(E)) (CAT#: HPAB-M0560-YC-F(E))
This product is a recombinant human antibody Fab fragment that recognizes LAM. The antibody possesses very high binding activity for LAM. The antibody was purified by affinity chromatography.
Specific Inquiry
WB
-1.jpg)
Figure 1 Anti-LAM Recombinant Antibody (clone A194-01); Fab Fragment (HPAB-M0560-YC-F(E)) in WB
Western blot analysis of HPAB-M0560-YC-F(E) was performed by loading Native M. Tuberculosis LAM-Antigen.
HPAB-M0560-YC-F(E) incubation concentration: 2 ng/μL.
The secondary antibody: HRP-conjugated Goat Anti-Human IgG ( H+L).
Lane 1: Reducing Antigen (0.5 μg)
Lane 2: Reducing Antigen (1 μg)
Lane 3: Reducing Antigen (2 μg)
ELISA
-2.jpg)
Figure 2 Anti-LAM Recombinant Antibody (clone A194-01); Fab Fragment (HPAB-M0560-YC-F(E)) in ELISA
ELISA analysis of HPAB-M0560-YC-F(E) was performed by coating with Native M. Tuberculosis LAM-Antigen. Then blocked with BSA and incubated with HPAB-M0560-YC-F(E). The HRP-conjugated Goat Anti-Human as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
SDS-PAGE
-3.jpg)
Figure 3 Anti-LAM Recombinant Antibody (clone A194-01); Fab Fragment (HPAB-M0560-YC-F(E)) in SDS-PAGE
SDS-PAGE analysis of HPAB-M0560-YC-F(E) in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 26 kDa and 25 kDa, respectively.
Specifications
- Host Species
- Human
- Type
- Human Fab
- Specificity
- LAM
- Species Reactivity
- M. Tuberculosis
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Please select product type
Recombinant Antibody
Fab Fragment Antibody
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABW-158 | Mouse Anti-LAM Recombinant Antibody (clone CS-35) | ELISA, IF, FC | Mouse IgG |
| PABC-542 | Recombinant Human Anti-LAM Antibody (P95C1) | ELISA, WB | Human IgM |
| HPAB-M0560-YC | Human Anti-LAM Recombinant Antibody (HPAB-M0560-YC) | ELISA, WB, Microarray, Block | Human IgG, κ |
| HPAB-M0561-YC | Human Anti-LAM Recombinant Antibody (HPAB-M0561-YC) | ELISA, WB, Microarray, Block | Human IgM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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