Mouse Anti-Lewis X Recombinant Antibody (clone 291-2G3-A) (CAT#: PABZ-085)

Recombinant Mouse Antibody (291-2G3-A) is capable of binding to Lewis X. A monoclonal antibody (mAb) 291-2G3-A that binds monomeric, dimeric, and trimeric Lex to gain detailed structural insight in the protein-Lex interaction.


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Figure 1 SPR Sensorgram, Binding Pattern of mAb 291-2G3-A with Lex-Related HSA-Neoglycoconjugates

Figure 1 SPR Sensorgram, Binding Pattern of mAb 291-2G3-A with Lex-Related HSA-Neoglycoconjugates

van Roon, A. M. M., Pannu, N. S., de Vrind, J. P., van der Marel, G. A., van Boom, J. H., Hokke, C. H., ... & Abrahams, J. P. (2004). Structure of an anti-Lewis X Fab fragment in complex with its Lewis X antigen. Structure, 12(7), 1227-1236.

Figure 2 Autocorrelation curves

Figure 2 Autocorrelation curves

Autocorrelation curves for BSA–LeX (15 μg/ml) incubated with 2 nM of PrA–Alexa 488, with PrA–Alexa 488 and 50 nM of control IgG1 or the same quantity of mab 291-2G3-A. Autocorrelation curves for PrA–Alexa 488 alone and PrA with control antibody decay almost identically. In case of the specific antibody (291-2G3-A), the autocorrelation curves have higher amplitude and a different rate of decay.

Mayboroda, O. A., van Remoortere, A., Tanke, H. J., Hokke, C. H., & Deelder, A. M. (2004). A new approach for fluorescence correlation spectroscopy (FCS) based immunoassays. Journal of biotechnology, 107(2), 185-192.

Figure 3 Immunoreactivity of each monoclonal antibody (MAb) analyzed by an indirect immunofluorescent assay and by immunodiffusion (ID)

Figure 3 Immunoreactivity of each monoclonal antibody (MAb) analyzed by an indirect immunofluorescent assay and by immunodiffusion (ID)

* The localization of fluorescence with sections of frozen livers from hamsters infected with Schistosoma mansoni (m) or S. haematobium (h) is summarized (+ = positive; ± = marginal; - = negative). Exs = excretory system; P-M = parenchyma and muscles; Teg = tegument; Mir = miracidia; Es = excretory/secretory antigen; Gran = granuloma; AWA = adult worm antigen; SEA = soluble egg antigen.

Nibbeling, H. A., Kahama, A. I., Van Zeyl, R. J., & Deelder, A. M. (1998). Use of monoclonal antibodies prepared against Schistosoma mansoni hatching fluid antigens for demonstration of Schistosoma haematobium circulating egg antigens in urine. The American journal of tropical medicine and hygiene, 58(5), 543-550.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Mouse
  • Derivation
  • Mouse
  • Type
  • Mouse IgG
  • Clone
  • 291-2G3-A
  • Applications
  • WB, IF, FuncS

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Lewis X; Le X

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

CAT Product Name Application Type
PABC-125 Mouse Anti-Lewis X Recombinant Antibody (clone 54-5C10-A) WB, FuncS Mouse IgG

scFv Fragment Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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