Hi-Affi™ Recombinant Rabbit Anti-MAP3K4 Monoclonal Antibody (DS2141AB) (CAT#: MOR-2141)
Recombinant Rabbit anti-MAP3K4 monoclonal antibody for WB, FC, IP, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/89_F12_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/89_F12_1_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_B_7_5.jpg
Figure 3 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_2_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_2_3.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_8_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_5_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_5_6.jpg
Figure 6 Lymph node
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_9_8.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085511-MAP3K4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085511-MAP3K4
Specifications
- Immunogen
- A synthetic peptide corresponding to residues in human MAP3K4 was used as immunogen. This antibody is predicted to detect splice isoform 2 based on sequence analysis.
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS2141AB
- Applications
- Used for immunoassay techniques such as: Western Blot; Flow Cytometry; Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
Related Resources
Endometrial Cancer
Throid Cancer
MAPK Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1176CT | Recombinant Mouse anti-Human MAP3K4 Monoclonal antibody (EML674) | IHC-P, WB | |
| VS3-FY2027 | Rabbit Anti-MAP3K4 Recombinant Antibody (clone R05-8A5) | WB, IHC-Fr, IHC-P, IF | Rabbit IgG |
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