Rabbit Anti-MAP3K4 Recombinant Antibody (clone R05-8A5) (CAT#: VS3-FY2027)
Clone R05-8A5, a rabbit recombinant monoclonal antibody that has been genetically engineered to specifically target human MAP3K4. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. Western blot demonstrated that R05-8A5 recognized a target protein with a molecular weight of 182 kDa.
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WB
Figure 1 Anti-MAP3K4 Antibody (VS3-FY2027) in WB.
The MAP3K4 expression in HL-60, U251 lysates were detected using the anti-MAP3K4 by western blot assay.
ICC
Figure 2 Anti-MAP3K4 Antibody (VS3-FY2027) in ICC.
Immunocytochemistry staining of MAP3K4 (green) in Hela using the anti-MAP3K4 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptides of human MAP3K4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAP3K4
- Species Reactivity
- Human
- Clone
- R05-8A5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 182 kDa, while the observed molecular weight stands at 200 kDa.
- Related Disease
- Breast Neuroendocrine Neoplasm and Neural Tube Defects
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase Kinase 4; MAPK/ERK Kinase Kinase 4; MAP Three Kinase 1; MEK Kinase 4; MAPKKK4; MEKK 4; MEKK4; MTK1
- Gene ID
- 4216
- UniProt ID
- Q9Y6R4
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9Y6R4)
Modification sites at neXtProt (NX_Q9Y6R4)
Glycosylation from GlyGen (Q9Y6R4) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_047274739.1; XP_054211427.1; NP_001278887.1; NP_001288001.2; NP_001350511.2
- Function
- Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
Endometrial Cancer
Throid Cancer
MAPK Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1176CT | Recombinant Mouse anti-Human MAP3K4 Monoclonal antibody (EML674) | IHC-P, WB |
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