Rabbit Anti-MAP3K4 Recombinant Antibody (clone R05-8A5)
CAT#: VS3-FY2027
Clone R05-8A5, a rabbit recombinant monoclonal antibody that has been genetically engineered to specifically target human MAP3K4. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. Western blot demonstrated that R05-8A5 recognized a target protein with a molecular weight of 182 kDa.
Tested Data
Gene Expression
WB
Figure 1 Anti-MAP3K4 Antibody (VS3-FY2027) in WB.
The MAP3K4 expression in HL-60, U251 lysates were detected using the anti-MAP3K4 by western blot assay.
ICC
Figure 2 Anti-MAP3K4 Antibody (VS3-FY2027) in ICC.
Immunocytochemistry staining of MAP3K4 (green) in Hela using the anti-MAP3K4 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/89_F12_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_B_7_5.jpg
Normal Tissue
Figure 3 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_2_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_5_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7625/28153_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000085511-MAP3K4
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Specifications
- Immunogen
- Synthetic peptides of human MAP3K4
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MAP3K4
- Species Reactivity
- Human
- Clone
- R05-8A5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 182 kDa, while the observed molecular weight stands at 200 kDa.
- Related Disease
- Breast Neuroendocrine Neoplasm and Neural Tube Defects
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Mitogen-Activated Protein Kinase Kinase Kinase 4; MAPK/ERK Kinase Kinase 4; MAP Three Kinase 1; MEK Kinase 4; MAPKKK4; MEKK 4; MEKK4; MTK1
- Gene ID
- 4216
- UniProt ID
- Q9Y6R4
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q9Y6R4)
Modification sites at neXtProt (NX_Q9Y6R4)
Glycosylation from GlyGen (Q9Y6R4) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_047274739.1; XP_054211427.1; NP_001278887.1; NP_001288001.2; NP_001350511.2
- Function
- Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
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Related Diseases
Endometrial Cancer
Throid Cancer
Related Signaling Pathways
MAPK Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP3K4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1176CT | Recombinant Mouse anti-Human MAP3K4 Monoclonal antibody (EML674) | IHC-P, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2141 | Hi-Affi™ Recombinant Rabbit Anti-MAP3K4 Monoclonal Antibody (DS2141AB) | WB, FC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4219 | Anti-MAP3K4 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4220 | Anti-Mouse MAP3K4 Immunohistochemistry Kit | IHC |
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