Anti-Human MC4R Therapeutic Antibody (1E8a) (CAT#: TAB-0733CL)

Recombinant monoclonal antibody 1E8a is a mouse monoclonal antibody that specifically binds to human MC4R and can be potentially used in the treatment of cachexia.

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ICC

Figure 1 Immunocytochemistry of HEK-293 cells transfected with hMC4R or hMC3R.

The specific membrane labeling was seen in HEK-293 cells expressing hMC4R when incubated with the mAb 1E8a.

Peter, J. C., Lecourt, A. C., Weckering, M., Zipfel, G., Niehoff, M. L., Banks, W. A., & Hofbauer, K. G. (2010). A pharmacologically active monoclonal antibody against the human melanocortin-4 receptor: effectiveness after peripheral and central administration. Journal of pharmacology and experimental therapeutics, 333(2), 478-490.

IP

Figure 2 The inhibition of hMC4R immunoprecipitation by mAb 1E8a using increasing concentration of the NT peptide.

Results are presented as percentage of inhibition of the
signal obtained without NT peptide in function of the [NT].
Mean=S.D. are calculated from three independent experiments.
n=5 measurements/experiment.

Peter, J. C., Lecourt, A. C., Weckering, M., Zipfel, G., Niehoff, M. L., Banks, W. A., & Hofbauer, K. G. (2010). A pharmacologically active monoclonal antibody against the human melanocortin-4 receptor: effectiveness after peripheral and central administration. Journal of pharmacology and experimental therapeutics, 333(2), 478-490.

WB

Figure 3 Western blot probed with an anti-His6 Ab that revealed the presence of the His6-tagged scFv 1E8a.

Purification of the scFv 1E8a by Ni-NTA chromatography.

Peter, J. C., Lecourt, A. C., Weckering, M., Zipfel, G., Niehoff, M. L., Banks, W. A., & Hofbauer, K. G. (2010). A pharmacologically active monoclonal antibody against the human melanocortin-4 receptor: effectiveness after peripheral and central administration. Journal of pharmacology and experimental therapeutics, 333(2), 478-490.


Specifications

  • Immunogen
  • Free peptide NT4
  • Host Species
  • Mouse
  • Specificity
  • Human
  • Clone
  • 1E8a
  • Applications
  • ICC, IP, WB
  • Related Disease
  • Cachexia

Applications

  • Application Notes
  • The MC4R antibody has been reported in applications of Immunocytochemistry, Immunoprecipitation, Western Blot.

Target

  • Alternative Names
  • MC4R; melanocortin 4 receptor; melanocortin receptor 4; MC4-R

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

See other products for "MC4R"


For research use only. Not intended for any clinical use.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
IAImmunoassay
IBImmunoblotting
ICCImmunocytochemistry
IDImmunodiffusion
IFImmunofluorescence
IHCImmunohistochemistry
IHC-FrImmunohistochemistry-Frozen
IHC-PImmunohistochemistry-Paraffin
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
Live cell imagingLive cell imaging
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
MultiplexMultiplex bead-based assay
NeutNeutralization
PPProtein Purification
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
TCTissue Culture
TBTurbidimetry
WBWestern Blot

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