Hi-Affi™ Recombinant Rabbit Anti-MDK Monoclonal Antibody (DS2198AB)
CAT#: MOR-2198
Recombinant Rabbit anti-MDK monoclonal antibody for ICC/IF, IHC-P, IP, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57126/996_H3_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in glandular cells and stroma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_B_4_3_selected.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_A_8_3.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_A_4_6.jpg
Normal Tissue
Figure 5 Endometrium
Cells in endometrial stroma Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_B_4_3.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (NTERA-2, REH, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110492-MDK
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Specifications
- Immunogen
- Synthetic peptide corresponding to residues on the C terminus (Human).
- Host Species
- Rabbit
- Derivation
- Phage display library screening
- Type
- IgG
- Species Reactivity
- Human
- Clone
- DS2198AB
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunohistochemistry (Paraffin); Immunoprecipitation; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Target
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Versatile MDK Antibody
This recombinant rabbit antibody (clone DS2198AB) is incredibly versatile. We successfully used it for ICC, IP, and Western Blotting to study Midkine. It specifically targets the C-terminus of the human protein.
Strong IHC Signal
We applied this antibody to paraffin-embedded tissue sections and observed strong, specific staining of MDK. The recombinant production ensures high specificity and sensitivity, making it a dependable reagent for our lab.
Q&As
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What part of the MDK protein is the immunogen derived from?
A: The immunogen used to generate this antibody is a synthetic peptide corresponding to residues on the C terminus of Human MDK (Midkine). This ensures specific recognition of the target protein in your assays.
-
What are the storage recommendations for this product?
A: We recommend storing the antibody at -20°C for long-term storage. For shorter periods of up to one month, it can be stored at 4°C. It is important to avoid freeze/thaw cycles to maintain the antibody's activity.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MDK"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0812CL | Human Anti-MDK Recombinant Antibody (TAB-0812CL) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0813CL | Mouse Anti-MDK Recombinant Antibody (TAB-0813CL) | ELISA, FC, Inhib | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0812CL-S(P) | Human Anti-MDK Recombinant Antibody; scFv Fragment (TAB-0812CL-S(P)) | ELISA | Humanized scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0813CL-S(P) | Mouse Anti-MDK Recombinant Antibody; scFv Fragment (TAB-0813CL-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0812CL-F(E) | Human Anti-MDK Recombinant Antibody; Fab Fragment (TAB-0812CL-F(E)) | ELISA | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0813CL-F(E) | Mouse Anti-MDK Recombinant Antibody; Fab Fragment (TAB-0813CL-F(E)) | ELISA, FC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1922MZ | Recombinant Mouse Anti-Human MDK Antibody (clone TD5) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| HPAB-AP735-YC | Human Anti-MDK Recombinant Antibody (clone EV3032) | ELISA, Neut | Human IgG1 |
| CAT | Product Name | Application | Type |
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| HPAB-AP735-YC-S(P) | Human Anti-MDK Recombinant Antibody (clone EV3032); scFv Fragment | ELISA, Neut | Human scFv |
| CAT | Product Name | Application | Type |
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| HPAB-1953-FY | Mouse Anti-MDK Recombinant Antibody (HPAB-1953-FY) | ELISA, Neut, Inhib | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| HPAB-1953-FY-F(E) | Mouse Anti-MDK Recombinant Antibody; Fab Fragment (HPAB-1953-FY-F(E)) | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1953-FY-S(P) | Mouse Anti-MDK Recombinant Antibody; scFv Fragment (HPAB-1953-FY-S(P)) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP735-YC-F(E) | Human Anti-MDK Recombinant Antibody (clone EV3032); Fab Fragment | ELISA, Neut | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0464U | Rabbit Anti-MDK Recombinant Antibody (clone 2G3) | ELISA, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS-0325-XY1328 | Anti-MDK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4321 | Anti-Human MDK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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