What is the specificity and host of this antibody?

This is a recombinant mouse monoclonal antibody (clone TD5) that specifically reacts with human MDK (Midkine). It is produced in CHO cells and is an IgG1 isotype, suitable for high-specificity detection.

What applications can this MDK antibody be used for?

This antibody has been validated for use in Enzyme-linked Immunosorbent Assay (ELISA). It serves as an excellent tool for quantifying human MDK levels in your experimental samples with high reliability.

Recombinant Mouse Anti-Human MDK Antibody (clone TD5)

CAT#: MOB-1922MZ

This antibody is a recombinant mouse monoclonal antibody which specifically reacts with human MDK.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57126/996_H3_2_selected.jpg

Normal Tissue Figure 2 IHC staining of human endometrium

Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in glandular cells and stroma.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_B_4_3_selected.jpg

Normal Tissue Figure 3 Colon

Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_A_8_3.jpg

Normal Tissue Figure 4 Testis

Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_A_4_6.jpg

Normal Tissue Figure 5 Endometrium

Cells in endometrial stroma Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10055/26957_B_4_3.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (NTERA-2, REH, SH-SY5Y)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000110492-MDK

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Specifications

Immunogen
Synthetic peptide (Human)

Host Species
Mouse

Type
Mouse antibody

Antibody Isotype
IgG1

Specificity
Human MDK

Species Reactivity
Human

Clone
TD5

Applications
ELISA

Target

Alternative Names
MDK; Human MDK

Gene ID
4192

UniProt ID
P21741

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Breast cancer biomarkers at key points during disease progression

Reliable Detection of MDK

The clone TD5 is a reliable recombinant mouse antibody for detecting human MDK. We used it in ELISA and obtained a linear standard curve. The specific reaction with human Midkine makes it an essential reagent for our growth factor research.

High Quality Production

We appreciate that this antibody is produced in CHO cells, ensuring high quality and consistency. It works perfectly in our immunoassays. The IgG1 isotype is compatible with our secondary antibodies, and we have had no issues with background signal.

Q&As

What is the specificity and host of this antibody?

A: This is a recombinant mouse monoclonal antibody (clone TD5) that specifically reacts with human MDK (Midkine). It is produced in CHO cells and is an IgG1 isotype, suitable for high-specificity detection.
What applications can this MDK antibody be used for?

A: This antibody has been validated for use in Enzyme-linked Immunosorbent Assay (ELISA). It serves as an excellent tool for quantifying human MDK levels in your experimental samples with high reliability.

View the frequently asked questions answered by Creative Biolabs Support.

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Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - MOB-1922MZ.pdf

MSDS

MSDS - MOB-1922MZ.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

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HPAB-1953-FY-F(E)	Mouse Anti-MDK Recombinant Antibody; Fab Fragment (HPAB-1953-FY-F(E))	ELISA	Mouse Fab

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HPAB-1953-FY-S(P)	Mouse Anti-MDK Recombinant Antibody; scFv Fragment (HPAB-1953-FY-S(P))	ELISA	Mouse scFv

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HPAB-AP735-YC-F(E)	Human Anti-MDK Recombinant Antibody (clone EV3032); Fab Fragment	ELISA, Neut	Human Fab

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