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    Hi-Affi™ Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody (DS2797AB) (CAT#: MOR-2797)

    Recombinant Rabbit anti-POU5F1 monoclonal antibody for FC, IHC-P, IP, WB, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line RT4
    Figure 1 IF staining of human cell line RT4
    (Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg
    Figure 2 IHC staining of human hippocampus
    Figure 2 IHC staining of human hippocampus
    (Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg
    Normal Tissue
    Figure 3 Cerebral cortex
    Figure 3 Cerebral cortex
    (Glial cells
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg
    Figure 4 Colon
    Figure 4 Colon
    (Endothelial cells
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Nuclear
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: Low
    Intensity: Weak
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg
    Figure 5 Liver
    Figure 5 Liver
    (Cholangiocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg
    Figure 6 Pancreas
    Figure 6 Pancreas
    (Exocrine glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Pancreatic endocrine cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg
    Figure 7 Kidney
    Figure 7 Kidney
    (Cells in glomeruli
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg
    Figure 8 Testis
    Figure 8 Testis
    (Cells in seminiferous ducts
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Cell line enriched (SuSa)
    Figure 10 RNA cell line category: Cell line enriched (SuSa)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular

    Specifications

    • Immunogen
    • Synthetic peptide, corresponding to amino acids 285-310 of Human Oct4.
    • Host Species
    • Rabbit
    • Derivation
    • Phage display library screening
    • Type
    • IgG
    • Species Reactivity
    • Human
    • Clone
    • DS2797AB
    • Applications
    • Used for immunoassay techniques such as: Flow Cytometry; Immunohistochemistry (Paraffin); Immunoprecipitation; Western Blot
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Purified with Absolute Mag™ Protein A Agarose
    • Purity
    • > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
    • Format
    • Liquid
    • Storage
    • Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.

    Target

    • Alternative Names
    • OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    Please select product type
    Rabbit Monoclonal Antibody Products IgG Antibody Products Cancer Stem Cell Biomarker Specific Antibody Products
    CAT Product Name Application Type
    MOR-0065-FY Rabbit Anti-POU5F1 Recombinant Antibody (clone AFY0036) FC, ICC, IHC-P, WB Rabbit IgG

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