Mouse Anti-POU5F1 Recombinant Antibody (clone 43H9)
CAT#: MOB-3985z
This product is a mouse antibody that recognizes human POU5F1. It can be used in the following applications: WB, IP, IHC, ELISA.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/58267/1518_H6_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human hippocampus
Immunohistochemical staining of human hippocampus shows strong nuclear positivity in glial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_7_4.jpg
Normal Tissue
Figure 6 Pancreas
Exocrine glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Pancreatic endocrine cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_3_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/25600/54340_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26380/57332_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enriched (SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000204531-POU5F1/subcellular
❮
❯
Specifications
- Immunogen
- Human POU class 5 homeobox 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Human POU5F1
- Species Reactivity
- Human
- Clone
- 43H9
- Applications
- WB, IP, IHC, ELISA
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western Blot, Immunoprecipitation Assay, Immunohistochemistry Assay, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- POU5F1; OTF4; Oct-4; POU Domain, Class 5, Transcription Factor 1; OCT3; OCT4; POU Class 5 Homeobox 1; Octamer-Binding Protein 4; Octamer-Binding Transcription Factor 3; POU Domain Class 5, Transcription Factor 1; OTF-3; POU-Type Homeodomain-Containing DNA
- UniProt ID
- Q01860
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for MOB-3985z. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "POU5F1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2215MZ | Recombinant Mouse Anti-Human POU5F1 Antibody (clone OSH2.2) | IHC-P | Mouse antibody |
| MRO-1126-CN | Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody (CBACN-411) | WB, IF, IHC, IP, ChIP | Rabbit IgG |
| MRO-1127-CN | Recombinant Mouse Anti-POU5F1 Monoclonal Antibody (A3-C11) | WB, IF | Mouse IgG1 |
| ZG-0236U | Mouse Anti-POU5F1 Recombinant Antibody (clone 2H41D8) | ELISA, WB, IHC, IF, FC | Mouse IgG2b |
| VS3-CJ41 | Mouse Anti-POU5F1 Recombinant Antibody (VS3-CJ41) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2797 | Hi-Affi™ Recombinant Rabbit Anti-POU5F1 Monoclonal Antibody (DS2797AB) | FC, IHC-P, IP, WB | IgG |
| MOR-0065-FY | Rabbit Anti-POU5F1 Recombinant Antibody (clone AFY0036) | FC, ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC84 | Mouse Anti-POU5F1 Recombinant Antibody (VS-0924-YC84) - Cancer Stem Cell Marker | IHC, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5587 | Anti-POU5F1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5588 | Anti-Mouse POU5F1 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: WB, IHC, FC, Cyt, ELISA
-3.jpg)
Application: ELISA, FC, WB, Block
Application: ELISA, FC, IF, WB
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: FC, IA, IF, IP, IHC, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
