Recombinant Mouse Anti-PfRH5 Antibody (QA1) (CAT#: PABL-302)

Figure 1 Investigation of the interaction of PfRH5ΔNL with a panel of mouse mAbs using ELISA.

Five mAbs that bind to PfRH5 were coated on a ELISA plate and probed using PfRH5ΔNL at concentrations of 12.5, 50, 200 or 800 ng/ml. Antibodies 9AD5, QA1 and QA5 interacted with PfRH5ΔNL while RB3 and 4BA7 did not. Indeed, RB3 and 4BA7 bind to the flexible N-terminus and the truncated loop, respectively, both features lacking in PfRH5ΔNL.

Wright, K. E., Hjerrild, K. A., Bartlett, J., Douglas, A. D., Jin, J., Brown, R. E.,... & Draper, S. J. (2014). Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Nature, 515(7527), 427.

Figure 2 Effects of anti-PfRH5 mAbs in the assay of GIA.

Anti-PfRH5 mouse mAbs were tested in the in vitro assay of GIA at a range of concentrations against (A) 3D7 clone P. falciparum parasites and (B) FVO parasites. Results show the mean of two experiments with triplicate wells.

Douglas, A. D., Williams, A. R., Knuepfer, E., Illingworth, J. J., Furze, J. M., Crosnier, C.,... & Alanine, D. G. (2014). Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5. The Journal of Immunology, 192(1), 245-258.